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首页> 外文期刊>Ophthalmic surgery, lasers & imaging: the official journal of the International Society for Imaging in the Eye >Preparation of donor lamellar tissue for deep lamellar endothelial keratoplasty using a microkeratome and artificial anterior chamber system: endothelial cell loss and predictability of lamellar thickness.
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Preparation of donor lamellar tissue for deep lamellar endothelial keratoplasty using a microkeratome and artificial anterior chamber system: endothelial cell loss and predictability of lamellar thickness.

机译：使用微型角膜刀和人工前房系统准备用于深层板层角膜移植的供体板层组织：内皮细胞损失和板层厚度的可预测性。

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BACKGROUND AND OBJECTIVE: To measure endothelial cell loss and predictability of lamellar thickness after preparing donor tissue for deep keratoplasty with an artificial anterior chamber and microkeratome. MATERIALS AND METHODS: A microkeratome set at a depth of 350 microm and a diameter of 9 mm was used to obtain ten lamellar lenticules from corneoscleral rims mounted in an artificial chamber. A punch trephine then was used to cut the donor tissue 7 mm in diameter. Specular microscopy was performed to evaluate endothelial cell density before the procedure, after cutting with the microkeratome, and after trephination. Pachymetry was performed to determine the predictability of lenticule thickness, before the procedure and after microkeratome incision. RESULTS: Mean post-microkeratome endothelial cell loss was 79 +/- 88 cells/mm2 and post-punch trephination was 85 +/- 94 cells/mm2. This represented a mean percentage loss of 3.2% and 3.5% for the respective steps of this procedure. Nine of the ten lenticules were cut within +/- 75 microm of the intended 350-microm thickness. CONCLUSIONS: Preparing donor lenticules for deep lamellar endothelial keratoplasty with a microkeratome with artificial chamber system caused a relatively small loss of endothelial cells (6.7% of the total) and a reproducible thickness. This may have advantages over manual preparation techniques.
机译：背景与目的：在准备供体组织用于人工前房和微角膜刀进行深层角膜移植后，测量内皮细胞的损失和层状厚度的可预测性。材料与方法：使用深度为350微米，直径为9毫米的微型角膜刀，从安装在人工室内的角膜巩膜缘获得十个片状微透镜。然后使用冲头环戊烷切割直径为7 mm的供体组织。在手术前，用微型角膜刀切开后以及在透色之后，进行镜面显微镜以评估内皮细胞密度。在进行微角膜刀切开术之前和之后，用测厚法确定微透镜厚度的可预测性。结果：微型角膜刀后内皮细胞平均损失为79 +/- 88细胞/ mm2，穿孔后透氨化为85 +/- 94细胞/ mm2。对于该过程的各个步骤，这表示平均损失百分比分别为3.2％和3.5％。十个小透镜中的九个被切成预期的350微米厚度的+/- 75微米。结论：用人工房系统的微型角膜刀为深层板状角膜内皮角膜移植术准备供体皮膜引起的内皮细胞损失相对较小（占总数的6.7％），厚度可再现。与手动准备技术相比，这可能具有优势。

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