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首页> 外文期刊>Cellular and Molecular Bioengineering >Quantification of Lacunar-Canalicular Interstitial Fluid Flow Through Computational Modeling of Fluorescence Recovery After Photobleaching
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Quantification of Lacunar-Canalicular Interstitial Fluid Flow Through Computational Modeling of Fluorescence Recovery After Photobleaching

机译：通过漂白后荧光恢复的计算模型对腔-小管间质液的流动进行定量

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Skeletal adaptation to mechanical loading has been widely hypothesized to involve the stimulation of osteocytes by interstitial fluid flow (IFF). However, direct investigation of this hypothesis has been difficult due in large part to the inability to directly measure IFF velocities within the lacunar-canalicular system. Measurements of fluorescence recovery after photobleaching (FRAP) within individual lacunae could be used to quantify lacunar-canalicular IFF when combined with mathematical modeling. In this study, we used a computational transport model to characterize the relationship between flow frequency (0.5-10 Hz), peak flow velocity (0-300 mu m/s), tracer diffusion coefficient (100-300 mu m(2)/s), and transport enhancement (i.e., (k/k(0)) - 1, where k and k(0) are the transport rates in the presence/absence of flow) during lacunar FRAP investigations. We show that this relationship is well described by a simple power law with frequency-dependent coefficients, and is relatively insensitive to variations in lacunar geometry. Using this power law relationship, we estimated peak IFF velocities in hindlimb mice subjected to intramedullary pressurization using values of k and k(0) previously obtained from ex vivo lacunar FRAP investigations. Together, our findings suggest that skeletal adaptation in hindlimb suspended mice subjected to dynamic intramedullary pressure occurred in the presence of IFF at levels associated with physiological loading.
机译：人们普遍假设骨骼对机械负荷的适应包括间质液流动（IFF）对骨细胞的刺激。但是，很难直接研究该假说，这在很大程度上是由于无法直接测量腔-小管系统内的IFF速度。当与数学建模相结合时，单个腔内光漂白后的荧光恢复（FRAP）的测量可用于量化腔-小管的IFF。在这项研究中，我们使用了计算传输模型来表征流动频率（0.5-10 Hz），峰值流速（0-300μm/ s），示踪剂扩散系数（100-300μm（2）/ s）和输运增强（即（k / k（0））-1，其中k和k（0）是在有腔流FRAP研究期间的传输速率）。我们表明，这种关系可以通过简单的幂定律很好地描述，并具有与频率相关的系数，并且对腔隙几何形状的变化相对不敏感。使用此幂律关系，我们使用先前从离体腔腔FRAP研究获得的k和k（0）值估算了接受髓内加压的后肢小鼠的峰值IFF速度。在一起，我们的发现表明，在存在动态髓内压的情况下，承受动态髓内压的后肢悬浮小鼠的骨骼适应发生在与生理负荷相关的水平。

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