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Automatic compensation of phase aberrations in digital holographic microscopy for living cells investigation by using spectral energy analysis

机译:通过使用光谱能量分析,在数字全息显微镜中自动补偿用于活细胞研究的相差

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摘要

Phase aberration compensation is crucial for quantitative phase-contrast imaging in digital holographic microscopy. In this paper, an automatic compensation method is proposed for living cells investigation in digital holographic microscopy. The phase aberrations are extracted and corrected automatically with a single hologram by using spectral energy analysis. Zernike polynomials are adopted to model the phase aberrations. The polynomial coefficients related to the amount of phase aberrations are calculated in a nonlinear optimization procedure, in which a spectral energy metric that places more weight on low- frequency components is maximized. The effectiveness of the proposed method is demonstrated with experimental result of mouse osteoblastic living cells.
机译:相差补偿对于数字全息显微镜中的定量相衬成像至关重要。本文提出了一种自动补偿方法,用于数字全息显微镜下的活细胞研究。通过使用光谱能量分析,可通过单个全息图自动提取并校正相差。采用Zernike多项式对相差进行建模。与相位像差量有关的多项式系数是在非线性优化程序中计算的,其中将对低频分量施加更多权重的频谱能量度量最大化。小鼠成骨细胞活细胞的实验结果证明了该方法的有效性。

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