...
首页> 外文期刊>Optics Letters >Tissue refractometry using Hilbert phase microscopy
【24h】

Tissue refractometry using Hilbert phase microscopy

机译:使用希尔伯特相显微镜的组织折光术

获取原文
获取原文并翻译 | 示例
           

摘要

We present, for the first time to our knowledge, quantitative phase images associated with unstained 5 (mu)m thick tissue slices of mouse brain, spleen, and liver. The refractive properties of the tissue are retrieved in terms of the average refractive index and its spatial variation. We find that the average refractive index varies significantly with tissue type, such that the brain is characterized by the lowest value and the liver by the highest. The spatial power spectra of the phase images reveal power law behavior with different exponents for each tissue type. This approach opens a new possibility for stain-free characterization of tissues, where the diagnostic power is provided by the intrinsic refractive properties of the biological structure. We present results obtained for liver tissue affected by a lysosomal storage disease and show that our technique can quantify structural changes during this disease development.
机译:我们首次了解到与小鼠大脑,脾脏和肝脏的未染色5微米厚组织切片相关的定量相位图像。根据平均折射率及其空间变化来获取组织的折射特性。我们发现平均折射率随组织类型而显着变化,因此大脑的特征在于最低值,肝脏的特征在于最高。相位图像的空间功率谱揭示了每种组织类型具有不同指数的幂律行为。这种方法为组织的无污染表征开辟了新的可能性,其中通过生物结构的固有折射特性提供了诊断能力。我们目前获得的结果受到溶酶体贮积病影响的肝组织,并表明我们的技术可以量化这种疾病发展过程中的结构变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号