Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

Millard AC.; Jin L.; Lewis A.; Loew LM.

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Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

机译：直接测量膜片固定细胞中膜染料产生二次谐波的电压灵敏度

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We report what. is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 nm. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells. (C) 2003 Optical Society of America. [References: 26]

机译：我们报告什么。据我们所知，是二次谐波产生的电压钳制电池的第一光学成像。对于膜染色的苯乙烯基染料di-4-ANEPPS，我们确定在850 nm的激发波长下，二次谐波产生的灵敏度为18％/ 100 mV。在荧光条件下，该灵敏度明显优于最佳的10％/ 100 mV，并且进一步确定了二次谐波产生对于活细胞膜电位功能成像的重要性。（C）2003年美国眼镜学会。 [参考：26]

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《Optics Letters》 |2003年第14期|共3页
作者
Millard AC.; Jin L.; Lewis A.; Loew LM.;
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正文语种 eng
中图分类光学;
关键词
Charge-shift probes; Microscopy; Fluorescence; Scattering; Tissue;

机译：电荷转移探针;显微镜;荧光;散射;组织;

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1. Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells [J] . Millard AC., Jin L., Lewis A., Optics Letters . 2003,第14期

机译：直接测量膜片固定细胞中膜染料产生二次谐波的电压灵敏度
2. Usefulness and Limitation of DiBAC4(3), a Voltage-Sensitive Fluorescent Dye, for the Measurement of Membrane Potentials Regulated by Recombinant Large Conductance Ca2+-Activated K+ Channels in HEK293 Cells [J] . Aki Yamada, Hiroshi Narita, Katsuhiko Muraki, Japanese Journal of Pharmacology . 2001,第3期

机译：DiBAC4（3），一种电压敏感的荧光染料，用于测量HEK293细胞中重组大电导Ca2 +激活的K +通道调节的膜电位的有用性和局限性
3. Cellular membranes that undergo cyclic changes in tension: Direct measurement of force generation by an in vitro contractile vacuole of Paramecium multimicronucleatum. [J] . Tani T, Allen RD, Naitoh Y Journal of Cell Science . 2001,第Pta4期

机译：经受张力周期性变化的细胞膜：草履虫多微核的体外收缩液泡直接测量力的产生。
4. Nonlinear properties of oriented purple membrane films derived from second-harmonic generation under picosecond excitation: prospect of electro-optical measurements of ultrafast photoelectric respon [C] . A.V. Sharkov, Tomas Gillbro Intermational conference on laser applications in life sciences . 1994

机译：在PICOSECOND激励下衍生自二次谐波产生的导向紫膜膜的非线性性能：超快光电谐波的电光测量前景
5. Interactions of light and matter for tissue characterization via second-harmonic generation imaging microscopy and light scattering measurements. [D] . Hall, Gunnsteinn. 2012

机译：光和物质的相互作用，用于通过二次谐波成像显微镜和光散射测量来表征组织。
6. Membrane Potential Measurements of Isolated Neurons Using a Voltage-Sensitive Dye [O] . Richard Fairless, Andreas Beck, Mykola Kravchenko, -1

机译：隔离神经元使用电压敏感染料的膜电位测量
7. Usefulness and Limitation of DiBAC4(3), a Voltage-Sensitive Fluorescent Dye, for the Measurement of Membrane Potentials Regulated by Recombinant Large Conductance Ca2+-Activated K+ Channels in HEK293 Cells [O] . Aki Yamada, Norikazu Gaja, Susumu Ohya, 2001

机译：Dibac4（3），电压敏感荧光染料的用性和限制，用于测量HEK293细胞中重组大导电Ca2 + -igtivated k +通道调节的膜电位的测量
8. Structure of Patch-Clamped Membranes in High Voltage Electron Microscopy. [R] . Ruknudin, A., Song, M. J., Auerbach, A., 1989

机译：高压电子显微镜中膜片钳结构的结构。

Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells

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