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首页> 外文期刊>Oral microbiology and immunology >Inhibiting effects of Streptococcus salivarius on competence-stimulating peptide-dependent biofilm formation by Streptococcus mutans.
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Inhibiting effects of Streptococcus salivarius on competence-stimulating peptide-dependent biofilm formation by Streptococcus mutans.

机译：唾液链球菌对变形链球菌刺激能力依赖性肽依赖性生物膜形成的抑制作用。

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INTRODUCTION: The effects of Streptococcus salivarius on the competence-stimulating peptide (CSP)-dependent biofilm formation by Streptococcus mutans were investigated. METHODS: Biofilms were grown on 96-well microtiter plates coated with salivary components in tryptic soy broth without dextrose supplemented with 0.25% sucrose. Biofilm formations were stained using safranin and quantification of stained biofilms was performed by measuring absorbance at 492 nm. RESULTS: S. mutans formed substantial biofilms, whereas biofilms of S. salivarius were formed poorly in the medium conditions used. Furthermore, in combination cultures, S. salivarius strongly inhibited biofilm formation when cultured with S. mutans. This inhibition occurred in the early phase of biofilm formation and was dependent on inactivation of the CSP of S. mutans, which is associated with competence, biofilm formation, and antimicrobial activity of the bacterium, and is induced by expression of the comC gene. Comparisons between the S. mutans clinical strains FSC-3 and FSC-3DeltaglrA in separate dual-species cultures with S. salivarius indicated that the presence of the bacitracin transport ATP-binding protein gene glrA caused susceptibility to inhibition of S. mutans biofilm formation by S. salivarius, and was also associated with the regulation of CSP production by com gene-dependent quorum sensing systems. CONCLUSION: It is considered that regulation of CSP by glrA in S. mutans and CSP inactivation by S. salivarius are important functions for cell-to-cell communication between biofilm bacteria and oral streptococci such as S. salivarius. Our results provide useful information for understanding the ecosystem of oral streptococcal biofilms, as well as the competition between and coexistence of multiple species in the oral cavity.
机译：简介：研究了唾液链球菌对变形链球菌形成能力刺激肽（CSP）依赖性生物膜形成的影响。方法：在96孔微量滴定板上生长生物膜，该微量滴定板上的唾液成分覆盖在胰蛋白酶大豆肉汤中，不含葡萄糖的0.25％蔗糖。使用番红花对生物膜形成进行染色，并通过测量492 nm的吸光度对染色的生物膜进行定量。结果：变形链球菌形成大量的生物膜，而唾液链球菌的生物膜在所用的中等条件下形成较差。此外，在组合培养中，唾液链球菌在与变形链球菌一起培养时强烈抑制生物膜的形成。这种抑制作用发生在生物膜形成的早期阶段，并且取决于变形链球菌的CSP失活，这与细菌的能力，生物膜形成和抗菌活性有关，并由comC基因的表达诱导。变形链球菌临床菌株FSC-3和FSC-3DeltaglrA在唾液链球菌分离的双物种培养中的比较表明，杆菌肽转运ATP结合蛋白基因glrA的存在引起抑制变形链球菌生物膜形成的敏感性。唾液链球菌，并且还与依赖于com基因的群体感应系统对CSP生产的调节有关。结论：glrA对变形链球菌的CSP调节和唾液链球菌的CSP失活被认为是生物膜细菌与唾液链球菌等口腔链球菌之间细胞间通讯的重要功能。我们的结果为了解口腔链球菌生物膜的生态系统以及口腔中多种物种之间的竞争和共存提供了有用的信息。

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