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首页> 外文期刊>Osteoarthritis and cartilage >Fibroblast growth factor-2 promotes the repair of partial thickness defects of articular cartilage in immature rabbits but not in mature rabbits.
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Fibroblast growth factor-2 promotes the repair of partial thickness defects of articular cartilage in immature rabbits but not in mature rabbits.

机译：成纤维细胞生长因子2促进未成熟兔关节软骨部分厚度缺损的修复，但在成熟兔中则不能。

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OBJECTIVE: To investigate cartilage response to fibroblast growth factor-2 (FGF-2) with increasing age in vivo, we examined the effect of FGF-2 on partial thickness defects of immature and mature rabbits. DESIGN: Sixty-nine Japanese white rabbits (34 immature rabbits, 35 mature rabbits) were examined. We made experimental partial thickness defects in articular cartilage of the knees. Then, we injected FGF-2 into the knees eight times, immediately after surgery and every 2 days for 2 weeks. A single dose of FGF-2 was 10 ng/0.1 ml or 100 ng/0.1 ml. In the control group, 0.1 ml saline was injected on the same time schedule. The rabbits were sacrificed at intervals following surgery that ranged from 2 to 48 weeks. The specimens were stained with toluidine blue and examined microscopically. We used a modified semiquantitative scale for evaluating the histological appearance of repair. RESULTS: In immature rabbits, the cartilage repair in the FGF-2 (100 ng)-treated group was significantly better than that ofthe other groups. The defects were almost completely repaired with chondrocytes that showed a round to polygonal morphology, and large amounts of extracellular matrix with intense metachromatic staining. In mature rabbits, however, there was apparently no effect from FGF-2 in either group. CONCLUSIONS: Application of FGF-2 facilitated cartilage repair in partial thickness defects in immature rabbits, but not in mature ones.
机译：目的：为了研究随着年龄增长而对成纤维细胞生长因子2（FGF-2）的软骨反应，我们研究了FGF-2对未成熟兔和成熟兔部分厚度缺损的影响。设计：检查了69只日本白兔（34只未成熟的兔子，35只成熟的兔子）。我们进行了膝关节软骨的实验性部分厚度缺陷。然后，我们在手术后立即和每2天注射2次，共8次，将FGF-2注入膝盖。 FGF-2的单剂量为10 ng / 0.1 ml或100 ng / 0.1 ml。在对照组中，在相同的时间表上注射0.1ml盐水。在2至48周的手术间隔后处死兔子。样品用甲苯胺蓝染色并进行显微镜检查。我们使用改良的半定量量表评估修复的组织学外观。结果：在未成熟兔中，FGF-2（100 ng）治疗组的软骨修复明显优于其他组。缺损几乎被软骨细胞完全修复，软骨细胞呈圆形至多边形形态，大量细胞外基质带有强烈的变色染色。然而，在成年兔子中，两组中的FGF-2显然都没有作用。结论：FGF-2的应用促进了未成熟兔部分厚度缺损的软骨修复，但在成熟兔中则没有。

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