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首页> 外文期刊>Osteoarthritis and cartilage >Laser scanning confocal arthroscopy of a fresh cadaveric knee joint.
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Laser scanning confocal arthroscopy of a fresh cadaveric knee joint.

机译：新鲜尸体膝关节的激光共聚焦关节镜检查。

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OBJECTIVE: Osteoarthritis (OA) inflicts an enormous burden upon sufferers and healthcare systems worldwide. Continuing efforts to elucidate the aetiology of OA have indicated the need for non-destructive methods of in vivo microstructural assessment of articular cartilage (AC). In this study, we describe the first use of a recently developed laser scanning confocal arthroscope (LSCA) to image the cartilage of a fresh frozen cadaveric knee from a patient with OA. DESIGN: Using an adaptation of the International Cartilage Repair Society (ICRS) joint mapping protocol, the joint was divided into three discrete regions (femoral condyle, patella and tibial plateau) for grading according to the ICRS (Outerbridge) system. The LSCA was used to generate images from each area within the three regions. Following imaging, the joint was sectioned and histology was performed on the corresponding sites with histological grading (modified-Mankin). RESULTS: Quantitative results of ICRS, LSCA and histological OA assessment were compared using intraclass correlation (ICC) and Pearson correlation analysis. The LSCA enabled visualisation of chondrocyte morphology and cell density, with classical OA changes such as chondrocyte clustering, surface fibrillation and fissure formation evident. Obvious qualitative similarities between LSCA images and histology were observed, with fair to moderate agreement (P<0.05) demonstrated between modalities. CONCLUSIONS: In this study, we have shown the viability of the LSCA for non-destructive imaging of the microstructure of OA knee cartilage. LSCA technology is potentially a valuable research and clinical tool for the non-destructive assessment of AC microstructure in early to late OA.
机译：目的：骨关节炎（OA）给全世界的患者和医疗系统带来巨大负担。阐明OA病因的持续努力表明，需要对关节软骨（AC）进行体内微结构评估的非破坏性方法。在这项研究中，我们描述了最新开发的激光扫描共聚焦关节镜（LSCA）首次用于对来自OA患者的新鲜冷冻尸体膝盖的软骨成像的图像。设计：采用国际软骨修复学会（ICRS）关节定位协议的改编版，将关节分为三个离散区域（股骨dy 、,骨和胫骨平台），以根据ICRS（外桥）系统进行分级。 LSCA用于从三个区域中的每个区域生成图像。成像后，将关节切开，并按照组织学分级（改良Mankin）在相应部位进行组织学检查。结果：使用类内相关（ICC）和皮尔逊相关分析比较了ICRS，LSCA和组织学OA评估的定量结果。 LSCA可以使软骨细胞形态和细胞密度可视化，并具有经典的OA变化，例如软骨细胞聚集，表面原纤维形成和裂缝形成。观察到LSCA图像和组织学之间明显的定性相似性，在模态之间显示出中等到中等的一致性（P <0.05）。结论：在这项研究中，我们已经证明了LSCA在OA膝关节软骨微结构的非破坏性成像中的可行性。 LSCA技术对于OA早期至晚期的AC显微结构的非破坏性评估而言，可能是有价值的研究和临床工具。

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