...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Osteoarthritis and cartilage >Possible involvement of the oxidized low-density lipoprotein/lectin-like oxidized low-density lipoprotein receptor-1 system in pathogenesis and progression of human osteoarthritis.
【24h】

Possible involvement of the oxidized low-density lipoprotein/lectin-like oxidized low-density lipoprotein receptor-1 system in pathogenesis and progression of human osteoarthritis.

机译：氧化的低密度脂蛋白/凝集素样氧化的低密度脂蛋白受体-1系统可能参与人类骨关节炎的发病机制和进展。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OBJECTIVE: Using human cartilage samples and cultured chondrocytes, to assess the possible involvement of oxidized low-density lipoprotein (ox-LDL) and lectin-like ox-LDL receptor-1 (LOX-1) in pathogenesis and progression of osteoarthritis (OA). METHODS: Thirty-two cartilage samples were obtained from 16 patients with knee OA, and 12 Control samples from six with femoral neck fracture. LOX-1 mRNA expressions in 12 OA and six Control samples were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemistry for ox-LDL and LOX-1 was performed in all samples. The histological OA grade was assessed with the modified Mankin score. The relative percentage of the ox-LDL and LOX-1 immunopositive chondrocytes was calculated in all samples. The effects of ox-LDL on cell viability in cultured human chondrocytes were investigated by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay and on proteoglycan synthesis by monitoring [35S] sulfate incorporation. RESULTS: There was a statistically significant difference between mean LOX-1/GAPDH (LOX-1/human glyceraldehyde-3-phosphate dehydrogenase) ratio of OA samples and that of Control samples (40.6%+/-10.3 and 11.9%+/-2.8, respectively, P<0.0001). The mean percentage of ox-LDL-positive cells was 23.0+/-15.7% in OA and 4.3+/-3.7% in Control cells (P=0.0002). The mean percentage of LOX-1-positive cells was 51.7+/-29.5% in OA and 10.0+/-8.1% in Control cells (P<0.0001). Both the ox-LDL immunoreactivity and the LOX-1 immunoreactivity were significantly correlated with the modified Mankin scores (R2=0.67 and 0.48, respectively; P<0.0001 for each). ox-LDL significantly reduced the human chondrocyte viability and proteoglycan synthesis, and pretreatment with anti-human LOX-1 monoclonal antibody reversed these effects. CONCLUSION: The ox-LDL/LOX-1 system may be involved in human OA.
机译：目的：使用人类软骨样品和培养的软骨细胞，评估氧化型低密度脂蛋白（ox-LDL）和凝集素样ox-LDL受体1（LOX-1）在骨关节炎（OA）的发病机理和进展中的可能作用。方法：从16例膝骨关节炎患者中获得32个软骨样品，并从6例股骨颈骨折中获得12个对照样品。通过逆转录-聚合酶链反应（RT-PCR）分析了12 OA和六个对照样品中的LOX-1 mRNA表达。在所有样品中对ox-LDL和LOX-1进行了免疫组织化学。用改良的Mankin评分评估组织学OA等级。计算所有样品中ox-LDL和LOX-1免疫阳性软骨细胞的相对百分比。通过3-（4,5-二甲基噻唑基-2）-2,5-二苯基溴化四溴甲烷（MTT）测定法研究ox-LDL对培养的人软骨细胞活力的影响，并通过监测[35S]硫酸盐的掺入来研究蛋白聚糖的合成。结果：OA样品与对照样品的平均LOX-1 / GAPDH（LOX-1 /人甘油醛-3-磷酸脱氢酶）之比有统计学意义（40.6％+ /-10.3和11.9％+ /-）分别为2.8和P <0.0001）。 OX-LDL阳性细胞的平均百分比在OA中为23.0 +/- 15.7％，在对照细胞中为4.3 +/- 3.7％（P = 0.0002）。 LOA-1阳性细胞的平均百分比在OA中为51.7 +/- 29.5％，在对照细胞中为10.0 +/- 8.1％（P <0.0001）。 ox-LDL免疫反应性和LOX-1免疫反应性均与改良的Mankin评分显着相关（分别为R2 = 0.67和0.48;每个P <0.0001）。 ox-LDL显着降低了人类软骨细胞的活力和蛋白聚糖的合成，抗人LOX-1单克隆抗体的预处理逆转了这些作用。结论：ox-LDL / LOX-1系统可能参与了人类OA。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号