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首页> 外文期刊>Osteoarthritis and cartilage >Evidence of chondrocyte repopulation in adult ovine articular cartilage following cryoinjury and long-term transplantation.
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Evidence of chondrocyte repopulation in adult ovine articular cartilage following cryoinjury and long-term transplantation.

机译：冻伤和长期移植后成年绵羊关节软骨中软骨细胞重新聚集的证据。

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OBJECTIVE: To characterize the response of articular chondrocytes to a specific cryoinjury that leads to cluster formation following long-term transplantation. DESIGN: Osteochondral dowels from 20 adult sheep were cryopreserved to optimize the recovery of chondrocytes immediately after thawing. The dowels were transplanted as allografts and observed at 3 and 12 months. Chondrocyte distribution and viability was assessed using paravital dyes after transplantation. Chondrocyte phenotype was assessed by in situ hybridization and immunohistochemistry to detect type II collagen. An anticentrosome antibody was used to identify cells undergoing cell cycle progression towards mitosis. RESULTS: All cryopreserved grafts showed the presence of spheroidal clusters of chondrocytes 1 year after transplantation while the host cartilage adjacent to the graft appeared morphologically normal. The average size of the clusters increased from four cells at 3 months to 12 cells at 1 year. The chondrocytes in the clusters displayed newly formed type II collagen protein and mRNA. Some cells within clusters were observed with two centrosomes, indicative of cells progressing through the S phase of the cell cycle. CONCLUSION: Adult articular chondrocytes retain the ability to repopulate the matrix, an ability which is demonstrated with this specific cryoinjury. This may be an initial stage of cartilage regeneration.
机译：目的：描述关节软骨细胞对特定冷冻损伤的反应，这种损伤导致长期移植后形成团簇。设计：将20只成年绵羊的软骨软骨销钉冷冻保存，以优化融化后立即恢复软骨细胞的功能。将销钉移植为同种异体移植，并在3个月和12个月时进行观察。移植后使用玻璃体染料评估软骨细胞的分布和生存能力。通过原位杂交和免疫组织化学评估软骨细胞表型以检测II型胶原。使用抗中心体抗体来鉴定经历细胞周期向有丝分裂发展的细胞。结果：所有冷冻保存的移植物在移植后1年都显示出软骨细胞的球形簇，而邻近移植物的宿主软骨在形态上是正常的。簇的平均大小从3个月的4个细胞增加到1年的12个细胞。簇中的软骨细胞显示新形成的II型胶原蛋白和mRNA。用两个中心体观察到簇内的一些细胞，表明细胞通过细胞周期的S期进行。结论：成年关节软骨细胞保留了重新填充基质的能力，这种特定的冷冻损伤证明了这一能力。这可能是软骨再生的初始阶段。

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