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首页> 外文期刊>Osteoarthritis and cartilage >Primary chondrocytes resist hydrostatic pressure-induced stress while primary synovial cells and fibroblasts show modified Hsp70 response.
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Primary chondrocytes resist hydrostatic pressure-induced stress while primary synovial cells and fibroblasts show modified Hsp70 response.

机译：原发性软骨细胞抵抗静水压力引起的压力，而原发性滑膜细胞和成纤维细胞显示出修饰的Hsp70反应。

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OBJECTIVE: During joint loading, chondrocytes in the articular cartilage are subjected to gradients of high compressive hydrostatic pressure (HP). In response to diverse chemical or physical stresses, heat shock genes are induced to express heat shock proteins (Hsps). This study sought to examine the role of Hsps in baroresistance in primary bovine chondrocytes and synovial cells, as well as in primary human fibroblasts. METHODS: Northern blotting was used to analyze the steady-state levels of hsp70 mRNA in the primary cells exposed to HP or heat stress. Hsp70 protein accumulation was analyzed by Western blotting, and the DNA-binding activity was examined by gel mobility shift assay. RESULTS: Primary bovine chondrocytes which have been adapted to live under pressurized conditions showed negligible Hsp70 response upon HP loading, whereas primary bovine synovial cells and human fibroblasts accumulated hsp70 mRNA and protein when subjected to HP. The response was initiated without activation of the heat shock transcription factor 1. Interestingly, pre-conditioning of the barosensitive fibroblasts with HP or heat shock reduced the Hsp70 response, indicating induction of baroresistance. CONCLUSION: This study suggests that Hsp70 can play an important role in the early stages of adaptation of cells to HP. Thus, the Hsp70 gene expression upon HP loading may serve as one indicator of the chondrocytic phenotype of the cells. This can be of use in the treatment of cartilage lesions.
机译：目的：在关节负荷过程中，关节软骨中的软骨细胞要经受高压缩静水压力（HP）的梯度作用。响应各种化学或物理压力，热激基因被诱导表达热激蛋白（Hsps）。这项研究试图检查Hsps在原代牛软骨细胞和滑膜细胞以及原代人成纤维细胞中的抗药性中的作用。方法：采用Northern印迹法分析HP或热应激下原代细胞中hsp70 mRNA的稳态水平。通过Western印迹分析Hsp70蛋白的积累，并通过凝胶迁移率测定法检测DNA结合活性。结果：适应高压条件下生活的原代牛软骨细胞在HP负荷下表现出可忽略的Hsp70反应，而原代牛滑膜细胞和人成纤维细胞在承受HP时积累了hsp70 mRNA和蛋白质。在没有激活热休克转录因子1的情况下开始了应答。有趣的是，用HP或热休克对压力敏感的成纤维细胞进行预处理可降低Hsp70应答，表明诱导了抗压力作用。结论：这项研究表明，Hsp70在细胞适应HP的早期阶段起着重要作用。因此，HP加载时的Hsp70基因表达可以作为细胞软骨细胞表型的一种指标。这可以用于治疗软骨损伤。

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