...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Osteoarthritis and cartilage >Cell deformation behavior in mechanically loaded rabbit articular cartilage 4 weeks after anterior cruciate ligament transection
【24h】

Cell deformation behavior in mechanically loaded rabbit articular cartilage 4 weeks after anterior cruciate ligament transection

机译：十字交叉韧带横断后4周机械负荷兔关节软骨的细胞变形行为

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: Chondrocyte stresses and strains in articular cartilage are known to modulate tissue mechanobiology. Cell deformation behavior in cartilage under mechanical loading is not known at the earliest stages of osteoarthritis. Thus, the aim of this study was to investigate the effect of mechanical loading on volume and morphology of chondrocytes in the superficial tissue of osteoarthritic cartilage obtained from anterior cruciate ligament transected (ACLT) rabbit knee joints, 4 weeks after intervention. Methods: A unique custom-made microscopy indentation system with dual-photon microscope was used to apply controlled 2 MPa force-relaxation loading on patellar cartilage surfaces. Volume and morphology of chondrocytes were analyzed before and after loading. Also global and local tissue strains were calculated. Collagen content, collagen orientation and proteoglycan content were quantified with Fourier transform infrared microspectroscopy, polarized light microscopy and digital densitometry, respectively. Results: Following the mechanical loading, the volume of chondrocytes in the superficial tissue increased significantly in ACLT cartilage by 24% (95% confidence interval (CI) 17.2-31.5, P < 0.001), while it reduced significantly in contralateral group tissue by -5.3% (95% CI -8.1 to -2.5, P = 0.003). Collagen content in ACLT and contralateral cartilage were similar. PG content was reduced and collagen orientation angle was increased in the superficial tissue of ACLT cartilage compared to the contralateral cartilage. Conclusions: We found the novel result that chondrocyte deformation behavior in the superficial tissue of rabbit articular cartilage is altered already at 4 weeks after ACLT, likely because of changes in collagen fibril orientation and a reduction in PG content. ? 2012 Osteoarthritis Research Society International.
机译：目的：已知软骨细胞的应力和应变可调节组织力学。在骨关节炎的最早期阶段，尚不知道机械载荷作用下软骨中的细胞变形行为。因此，本研究的目的是研究在干预后4周，机械载荷对从前交叉韧带横断（ACLT）兔膝关节获得的骨关节炎软骨表层组织软骨细胞的体积和形态的影响。方法：采用独特的定制双光子显微镜显微压痕系统在controlled骨软骨表面施加2 MPa的受控松弛力。加载前后分析软骨细胞的体积和形态。还计算了整体和局部组织应变。胶原蛋白含量，胶原蛋白取向和蛋白聚糖含量分别通过傅里叶变换红外光谱法，偏振光显微镜法和数字光密度法​​定量。结果：机械负荷后，ACLT软骨中浅表组织中的软骨细胞体积显着增加了24％（95％置信区间（CI）17.2-31.5，P <0.001），而对侧组组织中的显着降低了- 5.3％（95％CI -8.1至-2.5，P = 0.003）。 ACLT和对侧软骨中的胶原含量相似。与对侧软骨相比，ACLT软骨浅表组织中的PG含量降低，胶原蛋白取向角增加。结论：我们发现了一个新的结果，即ACLT后4周，兔关节软骨浅表组织中的软骨细胞变形行为已发生改变，这可能是由于胶原原纤维取向的改变和PG含量的降低。 ？ 2012年国际骨关节炎研究学会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号