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首页> 外文期刊>Osteoarthritis and cartilage >Glucosamine and chondroitin sulfate: biological response modifiers of chondrocytes under simulated conditions of joint stress.
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Glucosamine and chondroitin sulfate: biological response modifiers of chondrocytes under simulated conditions of joint stress.

机译：葡萄糖胺和硫酸软骨素：在模拟关节应激条件下软骨细胞的生物学反应调节剂。

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OBJECTIVE: To test the hypothesis that chondrocytes are more responsive to the chondroprotective agents, glucosamine (glcN) and chondroitin sulfate (CS), under in vitro conditions simulating in vivo joint stress. DESIGN: Synthetic and anticatabolic activities of bovine articular cartilage were assayed using 35-sulfate labeling and assaying the specific activity of glycosaminoglycans (GAGs) under the conditions of enzyme-induced matrix depletion, heat stress, mechanical compression and cytokine stress. RESULTS: The response of cartilage to simulated conditions of in vivo stress varies, depending on the type stress and age of the animal. Cartilage from aged animals was more responsive to stress and to glcN and CS. Pronase-induced matrix depletion and mechanical stress increased proteoglycan synthetic activity. Exposure to glcN and CS significantly enhanced this stress response from 85 to 191% and from 40 to 1000%, respectively. Heat stress and stromelysin digestion decreased synthetic activity, which wasreversed or normalized on exposure to glcN and CS. Cartilage from young joints was somewhat refractory to the level of stress imposed and to treatment with glcN and CS. CONCLUSION: The metabolic response of cartilage from aged animals to glcN and CS under simulated conditions of in vivo stress is significantly greater than that seen in nonstressed or young tissue. By enhancing the "protective" metabolic response of chondrocytes to stress, glcN and CS may improve its ability for repair and regeneration. These observations suggest that these compounds function as biological response modifiers (BRMs), agents which boost natural protective responses of tissues under adverse environmental conditions.
机译：目的：在体外模拟体内关节应激的条件下，测试软骨细胞对软骨保护剂，葡萄糖胺（glcN）和硫酸软骨素（CS）的反应更强的假设。设计：使用35硫酸盐标记法检测牛关节软骨的合成和抗催化活性，并在酶诱导的基质耗竭，热应激，机械压缩和细胞因子应激的条件下测定糖胺聚糖（GAG）的比活性。结果：软骨对体内应激模拟条件的反应各不相同，具体取决于动物的应激类型和年龄。老年动物的软骨对压力以及glcN和CS的反应更大。链淀粉酶诱导的基质耗竭和机械应力增加了蛋白聚糖的合成活性。暴露于glcN和CS分别显着增强了这种应激反应，分别从85％增至191％和40％至1000％。热应激和溶酶溶酶消化降低了合成活性，当暴露于glcN和CS时合成活性被逆转或归一化。年轻关节的软骨对施加的压力以及glcN和CS的治疗均具有一定的抵抗力。结论：在模拟的体内应激条件下，老年动物对glcN和CS的软骨的代谢反应显着大于未受压或年轻的组织。通过增强软骨细胞对应激的“保护性”代谢反应，glcN和CS可以改善其修复和再生能力。这些观察结果表明，这些化合物起着生物反应调节剂（BRM）的作用，可在不利的环境条件下增强组织的自然保护反应。

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