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首页> 外文期刊>Osteoarthritis and cartilage >Early diagnosis of osteoarthritis using cathepsin B sensitive near-infrared fluorescent probes.
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Early diagnosis of osteoarthritis using cathepsin B sensitive near-infrared fluorescent probes.

机译：使用组织蛋白酶B敏感的近红外荧光探针早期诊断骨关节炎。

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OBJECTIVE: Osteoarthritis is currently diagnosed utilizing X-ray and MRI-techniques, both of which are based on the morphological changes of tissue. However, once changes are detected, the tissue has an irreversible defect. This study investigates early diagnosis of OA on a molecular basis using a recently developed cathepsin B sensitive near-infrared (NIR) fluorescent probe. METHOD: Twelve male nude mice were induced osteoarthritis by intra-articular injection of collagenase (1.0%, w/v) into the right knee joint. The left knee joint served as the negative control. The cathepsin B NIR probe is activated by arthritis-associated cathepsin B, thus resulting in the emission of an intensive NIR fluorescence signal which can be detected in vivo. NIR fluorescence signals were acquired on an optical imaging system using an excitation wavelength of 610-650 nm and an emission wavelength of 680-720 nm. RESULTS: Mild to moderate degenerative cartilage was observed 1 month after collagenase injection. NIR fluorescence imaging of mice showed approximately a 3-fold difference in signal intensity between osteoarthritic and normal joints 24 h after intravenous injection of the reporter probe. Immunohistochemical evaluation also revealed cathepsin B expression in the arthritic lesion of femorotibial joints, and not in the control contra-lateral knee joints. CONCLUSION: As the cathepsin B activatable NIR fluorescent imaging showed a significant difference between the osteoarthritic and normal joints, the cathepsin B activatable NIR fluorescent probe thus offers a potential new imaging technology for early OA diagnosis.
机译：目的：目前利用X射线和MRI技术诊断骨关节炎，这两种技术都是基于组织的形态变化。但是，一旦检测到变化，组织将具有不可逆的缺陷。这项研究使用最近开发的组织蛋白酶B敏感的近红外（NIR）荧光探针在分子基础上研究OA的早期诊断。方法：通过向右膝关节内关节内注射胶原酶（1.0％，w / v），使十二只雄性裸鼠诱发骨关节炎。左膝关节作为阴性对照。组织蛋白酶B NIR探针被关节炎相关的组织蛋白酶B激活，从而导致发出强烈的NIR荧光信号，该信号可在体内检测到。在光学成像系统上使用610-650 nm的激发波长和680-720 nm的发射波长采集NIR荧光信号。结果：注射胶原酶1个月后观察到轻度至中度的变性软骨。小鼠的NIR荧光成像显示，静脉注射报告探针后24小时，骨关节炎和正常关节之间的信号强度大约有3倍的差异。免疫组织化学评估还显示组织蛋白酶B在股骨关节的关节炎病变中表达，而在对照对侧膝关节中则不表达。结论：由于组织蛋白酶B激活的NIR荧光成像显示出骨关节炎与正常关节之间的显着差异，因此组织蛋白酶B激活的NIR荧光探针为早期OA诊断提供了一种潜在的新成像技术。

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