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首页> 外文期刊>Optica applicata >SU-8 photoresist as material of optical passive components integrated with analytical microsystems for real-time polymerase chain reaction
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SU-8 photoresist as material of optical passive components integrated with analytical microsystems for real-time polymerase chain reaction

机译：SU-8光刻胶作为光学无源组件的材料，与分析微系统集成在一起，用于实时聚合酶链反应

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Rapid development of analytical microsystems, also often called as labs-on-a-chip and used to perform various chemical and biochemical analysis with nano- and pico-volumes of the sample, has been observed for almost two decades. Successful application of analytical microsystems in medical and veterinary practices is relevant to many factors but one of them is low price of the disposable microsystem – often in the form of a chip. The chips are made of cheap materials, for example, polymers. One of such material is a negative photoresist SU-8. It is characterized by biocompatibility and possibility of easy fabrication of various three-dimensional fluidic micro-structures. Moreover, SU-8 is transparent for visible light. It makes SU-8 attractive material for labs-on-a-chip dedicated for genetic material analysis by real-time polymerase chain reaction (PCR). In this paper, we present the results of investigations of the influence of PCR-like temperature profiling on the transmittance spectra. The autofluorescence effect of SU-8 illuminated with various lasers has also been investigated as one of the factors limiting sensitive fluorescence readout. The results obtained showed that SU-8 can be successfully applied as labs-on-a-chip material but, due to high SU-8 autofluorescence, red-line fluorochromes are preferred when high-sensitivity fluorescence detection is required.
机译：分析微系统的快速发展，通常被称为芯片实验室，用于对样品的纳米和皮克体积进行各种化学和生化分析，已经观察了近二十年。分析微系统在医学和兽医实践中的成功应用与许多因素有关，但其中之一是一次性微系统的价格低廉-通常采用芯片形式。芯片由便宜的材料制成，例如聚合物。这种材料之一是负性光刻胶SU-8。它的特点是具有生物相容性，并且易于制造各种三维流体微结构。此外，SU-8对可见光透明。它使SU-8有吸引力的材料可用于芯片实验室，用于通过实时聚合酶链反应（PCR）进行遗传材料分析。在本文中，我们介绍了类似PCR的温度分布图对透射光谱的影响的研究结果。还已经研究了用各种激光照射的SU-8的自发荧光效应，这是限制敏感荧光读数的因素之一。所得结果表明，SU-8可以成功用作芯片实验室材料，但是由于SU-8自发荧光较高，因此在需要高灵敏度荧光检测时，首选红线荧光染料。

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