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首页> 外文期刊>Otolaryngology--head and neck surgery: official journal of American Academy of Otolaryngology-Head and Neck Surgery >Mucin 5 subtype AC expression and upregulation in the nasal mucosa of allergic rhinitis rats.
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Mucin 5 subtype AC expression and upregulation in the nasal mucosa of allergic rhinitis rats.

机译：变应性鼻炎大鼠鼻黏膜中黏蛋白5亚型AC的表达和上调。

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摘要

To elucidate the functions of nuclear factor kappa B (NF-κB) in mucin hypersecretion in allergic rhinitis (AR), we examined the in vivo effects of an NF-κB inhibitor, ammonium pyrrolidine dithiocarbamate (PDTC), on mucin 5 subtype AC (MUC5AC) expression in the nasal mucosa of ovalbumin-sensitized rats.Randomized animal study.Academic medical center.Sprague Dawley rats were randomized into a control group (group A), an AR model group (group B), and an AR model treated with an NF-κB inhibitor (group C). Rats in groups B and C were sensitized systemically and locally by ovalbumin injection and inhalation, whereas group A was treated with normal saline in place of ovalbumin. Pyrrolidine dithiocarbamate (100 mg/kg/d) was given to group C by intraperitoneal injection for 5 days. NF-κBp65, MUC5AC, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were detected by immunohistochemistry, Western blotting, enzyme-linked immunosorbent assay, or real-time polymerase chain reaction.NF-κB was activated in group B, and significant NF-κBp65 protein was expressed in the nucleus of cells from the nasal mucosa, resulting in upregulated transcription from TNF-α and IL-6 genes, as well as increased contents of TNF-α and IL-6 in the nasal lavage fluids. Pyrrolidine dithiocarbamate inhibited nuclear localization of NF-κBp65 and subsequent downregulation of the transcription and secretion of TNF-α and IL-6. MUC5AC was upregulated in group B but reduced in a time-dependent manner after inhibition of NF-κB activation.NF-κB activation might induce MUC5AC hypersecretion in AR rats by inflammatory cytokines TNF-α and IL-6.
机译：为了阐明核因子κB（NF-κB）在变应性鼻炎（AR）黏蛋白过度分泌中的功能，我们检查了NF-κB抑制剂吡咯烷二硫代氨基甲酸铵（PDTC）对黏蛋白5亚型AC（卵清蛋白致敏大鼠鼻黏膜中MUC5AC的表达。随机动物研究。医学医学中心.Sprague Dawley大鼠随机分为对照组（A组），AR模型组（B组）和经ADR治疗的AR模型NF-κB抑制剂（C组）。 B和C组的大鼠通过卵清蛋白注射和吸入全身和局部致敏，而A组则用生理盐水代替卵清蛋白治疗。通过腹膜内注射将吡咯烷二硫代氨基甲酸酯（100mg / kg / d）给予C组5天。 NF-κBp65，MUC5AC，肿瘤坏死因子（TNF）-α和白介素（IL）-6通过免疫组织化学，Western印迹，酶联免疫吸附法或实时聚合酶链反应检测，NF-κB在B组在鼻黏膜细胞核中表达明显的NF-κBp65蛋白，导致TNF-α和IL-6基因的转录上调，并且TNF-α和IL-6的含量增加。鼻灌洗液。吡咯烷二硫代氨基甲酸酯抑制NF-κBp65的核定位并随后下调TNF-α和IL-6的转录和分泌。 B组中MUC5AC表达上调，但在NF-κB激活抑制后呈时间依赖性降低。NF-κB激活可能通过炎性细胞因子TNF-α和IL-6诱导AR大鼠MUC5AC过度分泌。

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