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首页> 外文期刊>Otolaryngology--head and neck surgery: official journal of American Academy of Otolaryngology-Head and Neck Surgery >Topographic gene expression in the sinonasal cavity of patients with chronic sinusitis with polyps.
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Topographic gene expression in the sinonasal cavity of patients with chronic sinusitis with polyps.

机译：慢性鼻窦炎伴息肉患者的鼻窦腔内地形基因表达。

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OBJECTIVE: To determine whether variations in gene expression exist at multiple subsites along the sinonasal tract in patients with chronic sinusitis with polyps and in healthy controls. STUDY DESIGN: Prospective, controlled study. SETTING: Academic medical center. SUBJECTS AND METHODS: Tissue expression levels of 5 genes, previously found to be characteristic of ethmoid polyps, were measured using real-time quantitative polymerase chain reaction in 100 sinonasal tissue samples. Specimens harvested from 5 regions--the ethmoid sinus, septum, inferior turbinate, middle turbinate, and lateral nasal wall--in 10 patients with chronic sinusitis and ethmoid polyps were compared to tissue from similar regions in 10 control patients without sinusitis. Western blot analysis was performed to validate differential gene expression at the protein level. RESULTS: Gene expression levels of ethmoid polyps differed significantly from those of healthy ethmoid mucosa, as well as tissue from 4 surrounding anatomical sites in both patients with chronic sinusitis and controls. Alterations specific to the polyp tissue included downregulated genes, prolactin-induced protein (fold change 377.2 +/- 169.0, P < .0001), and zinc alpha2-glycoprotein (fold change 72.1 +/- 26.5, P < .0001), as well as upregulated genes, met proto-oncogene (fold change 2.5 +/- 0.7, P = .029), and periostin (fold change 7.5 +/- 3.4, P = .003). No significant differences in gene expression was found for neurabin 2 (fold change 1.0, P = .99). CONCLUSION: The transcriptional pattern of ethmoid polyps appears to be unique compared with other subsites in the sinonasal cavity of patients with chronic sinusitis. Care must be taken when collecting specimens for molecular studies of the sinonasal tract to differentiate polyp from nonpolyp tissue in chronic sinusitis.
机译：目的：确定患有鼻息肉的慢性鼻窦炎患者和健康对照者的鼻窦道多个亚位点基因表达是否存在变异。研究设计：前瞻性对照研究。地点：学术医学中心。研究对象和方法：使用实时定量聚合酶链反应在100例鼻窦组织样本中测量了5种基因的组织表达水平，以前发现这是筛骨息肉的特征。在10例慢性鼻窦炎和筛窦息肉患者中，从5个区域（筛窦，隔，下鼻甲，中鼻甲和鼻外侧壁）收集的标本与来自10个非鼻窦炎对照患者相似区域的组织进行了比较。进行蛋白质印迹分析以验证蛋白质水平的差异基因表达。结果：在慢性鼻窦炎患者和对照组中，筛窦息肉的基因表达水平与健康筛窦粘膜以及周围4个解剖部位组织的表达水平存在显着差异。息肉组织特有的改变包括下调的基因，催乳素诱导的蛋白质（倍数变化377.2 +/- 169.0，P <.0001）和锌α2-糖蛋白（倍数变化72.1 +/- 26.5，P <.0001），以及上调的基因，遇到原癌基因（倍数变化2.5 +/- 0.7，P = .029）和骨膜蛋白（倍数变化7.5 +/- 3.4，P = .003）。神经氨酸蛋白2的基因表达没有发现显着差异（倍数变化1.0，P = 0.99）。结论：与慢性鼻窦炎患者鼻窦腔中的其他亚位相比，筛窦息肉的转录方式似乎是独特的。收集标本进行鼻窦道分子研究时必须小心，以区分慢性鼻窦炎中息肉与非息肉组织。

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