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Atomic force microscopy and tridimensional topography analysis of human enamel after resinous infiltration and storage in water

机译:树脂渗透入水中并储存后的人类牙釉质的原子力显微镜和三维形貌分析

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Objectives: To evaluate the effect of water storage on surface roughness (Ra) of human enamel after treatment with resin infiltrant and fissure sealant, by utilizing atomic force microscopy (AFM) and microtomography. Methods: This study was conducted after registration and ethical approval clarification at the College of Dentistry Research Center, King Saud University, Riyadh, Kingdom of Saudi Arabia between January 2011 and August 2011. Thirty enamel surface specimens were prepared from caries-free human premolar teeth. Specimens were divided into 3 groups: Group I, was the control; Group II, a resin infiltrant (Icon?) was applied on the enamel surfaces; and Group III, the teeth were treated with fissure sealant (SealRite?). All specimens were stored in distilled water for 6 months and then, subjected to AFM Veeco CP11 1.2 analysis. A few specimens were scanned by skyscan-1072-x-ray microtomography. The Ra mean readings were recorded and statistical analysis was performed with the Statistical Package for Social Sciences Version 16 at the significance level of p<0.05. Results: No significant differences in the mean Ra were recorded among the 3 groups, (Group I = 0.21±0.057), (Group II = 0.23±0.075), and (Group III = 0.20±0.039) at p=0.747. The AFM images of enamel surface show thin and inhomogeneous Icon resin in Group II, meanwhile, the SealRite in Group III showed a homogeneous layer in all specimens. The microtomography supported the findings of the AFM images. Conclusion: The persistence of the SealRite in all specimens revealed its low solubility in water and its protective effect on enamel surface.
机译:目的:利用原子力显微镜(AFM)和显微断层照相术,评估贮水对树脂浸润剂和裂隙密封剂处理后人牙釉质表面粗糙度(Ra)的影响。方法:本研究是在2011年1月至2011年8月之间,在沙特阿拉伯利雅得金沙特大学牙科学院研究中心注册并获得伦理学批准后进行的。从无龋的人类前磨牙制备了30个搪瓷表面标本。 。标本分为三组:第一组为对照组;第二组为对照组。第II组,将树脂浸润剂(Icon?)涂在瓷釉表面;和第三组,用裂隙密封剂(SealRite?)治疗牙齿。将所有样品在蒸馏水中保存6个月,然后进行AFM Veeco CP11 1.2分析。通过skyscan-1072-x射线显微断层扫描术扫描了一些标本。记录Ra平均值,并使用统计软件包(社会科学第16版)在p <0.05的显着性水平下进行统计分析。结果:在p = 0.747时,三组(I组= 0.21±0.057),(II组= 0.23±0.075)和(III组= 0.20±0.039)之间的平均Ra没有显着差异。牙釉质表面的原子力显微镜图像显示第二组中稀薄且不均匀的Icon树脂,而第三组中的SealRite在所有样品中均显示出均匀的层。显微断层扫描支持AFM图像的发现。结论:SealRite在所有样品中的持久性表明其在水中的溶解度低,并且对牙釉质表面具有保护作用。

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