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首页> 外文期刊>Biological chemistry >Dynamics of septin ring and collar formation in Saccharomyces cerevisiae.
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Dynamics of septin ring and collar formation in Saccharomyces cerevisiae.

机译：酿酒酵母中Septin环和项圈形成的动力学。

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摘要

Although the septin ring and collar in budding yeast were described over 20 years ago, there is still controversy regarding the organization of septin filaments within these structures and about the way in which the ring first forms and about how it converts into a collar at the mother-bud neck. Here we present quantitative analyses of the recruitment of fluorescently-tagged septins to the ring and collar through the cell cycle. Septin ring assembly began several minutes after polarity establishment and this interval was longer in daughter than in mother cells, suggesting asymmetric inheritance of septin regulators. Septins formed an initial faint and irregular ring, which became more regular as septins were recruited at a constant rate. This steady rate of septin recruitment continued for several minutes after the ring converted to a collar at bud emergence. We did not detect a stepwise change in septin fluorescence during the ring-to-collar transition. After collar formation, septins continued to accumulate at the bud neck, though at a reduced rate, until the onset of cytokinesis when the amount of neck-localized septins rapidly decreased. Implications for the mechanism of septin ring assembly are discussed.
机译：尽管二十多年前就已经描述了发芽酵母中的Septin环和环，但是关于这些结构中Septin细丝的组织以及环首次形成的方式以及如何在母体上转化为环的问题仍然存在争议芽脖子。在这里，我们提出了通过细胞周期将荧光标记的Septin募集到环和颈圈的定量分析。极性确定后几分钟，Septin环组装就开始了，子代中的间隔比母细胞中的更长，这表明Septin调节子的遗传不对称。 Septins形成了最初的模糊且不规则的环，随着Septins以恒定的速率被吸收，它变得更加规则。在芽出现时，环转化为颈圈后，这种稳定的Septin募集速度持续了几分钟。我们没有检测到环到颈过渡过程中Septin荧光的逐步变化。领形成后，隔膜仍继续在芽颈处积聚，尽管速率降低，直到细胞分裂开始，当颈部局部隔膜的数量迅速减少时。讨论了Septin环组装机制的含义。

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