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首页> 外文期刊>Molecular Microbiology >Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis
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Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis

机译：细菌中群体反应控制的基因和过程的保守性：枯草芽孢杆菌中群体感应转录因子ComA控制的基因的表征

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摘要

Quorum or diffusion responses in bacteria are mediated by secreted signalling molecules that accumulate extracellularly as cultures grow to high density. The regulatory response to these signalling molecules can result in dramatic changes in gene expression. In Bacillus subtilis, a quorum response is mediated by a secreted 10-amino-acid modified peptide (ComX pheromone) that activates a receptor histidine kinase (ComP) that activates a response regulator transcription factor (ComA). We have used DNA microarrays to identify genes controlled by the ComX-ComP-ComA quorum-sensing pathway. We found that ComX, ComP and ComA affect the same set of genes, indicating that the kinase ComP is the only receptor for the signalling molecule ComX, and that ComA is the only transcription factor activated directly by ComP, under the conditions tested. Expression of over 20 genes appears to be controlled directly by this signalling pathway, and expression of over 150 additional genes, including those involved in competence development, appears to be controlled indirectly. The genes affected appear to have three general functions: (i) to co-ordinate physiological changes involved in developmental pathways, (ii) to produce extracellular products under conditions in which high concentrations of product are needed to be effective and (iii) to enhance survival, growth and colonization under conditions of crowding or limited diffusion. Many of the genes and processes controlled by the quorum response in B. subtilis are also regulated by quorum sensing in Gram-positive and Gram-negative bacteria. The quorum-sensing signalling molecules and regulatory proteins are quite different between Gram-positives and Gram-negatives and the convergent physiological regulation of similar genes and processes indicate the important and conserved nature of the quorum response.
机译：细菌中的定额或扩散反应是由分泌的信号分子介导的，这些信号分子随着培养物生长到高密度而在细胞外积累。对这些信号分子的调节反应可能导致基因表达发生巨大变化。在枯草芽孢杆菌中，群体反应由分泌的10个氨基酸修饰的肽（ComX信息素）介导，该肽激活受体组氨酸激酶（ComP），后者激活反应调节因子转录因子（ComA）。我们已经使用DNA微阵列来识别由ComX-ComP-ComA群体感应途径控制的基因。我们发现ComX，ComP和ComA影响同一组基因，表明在测试条件下，激酶ComP是信号分子ComX的唯一受体，并且ComA是ComP直接激活的唯一转录因子。超过20个基因的表达似乎直接受此信号通路控制，而超过150个其他基因（包括与能力发展有关的基因）的表达似乎受到间接控制。受影响的基因似乎具有三个一般功能：（i）协调涉及发育途径的生理变化，（ii）在需要高浓度产品有效的条件下产生细胞外产物，以及（iii）增强在拥挤或扩散受限的条件下存活，生长和定殖。枯草芽孢杆菌的群体应答控制的许多基因和过程也受到革兰氏阳性和革兰氏阴性细菌的群体感应的调节。革兰氏阳性和革兰氏阴性之间的群体感应信号分子和调节蛋白完全不同，相似基因和过程的趋同生理调节表明群体反应的重要和保守性质。

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