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首页> 外文期刊>Scientia horticulturae >Isolation and expression analysis of CiNIP5, a citrus boron transport gene involved in tolerance to boron deficiency.
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Isolation and expression analysis of CiNIP5, a citrus boron transport gene involved in tolerance to boron deficiency.

机译：CiNIP5的分离和表达分析，CiNIP5是一种对硼缺乏症的耐受性的柑橘硼转运基因。

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摘要

It was reported that AtNIP5;1 is responsible for boron (B) uptake into the root under B-deficient conditions. In this study, we cloned an NIP5-like gene (named as CiNIP5) from Trifoliate orange. The full length of CiNIP5 was 1470 bp in which 903 bp formed the open reading frame, coding a protein of 300 amino acids. Sequence analysis showed that CiNIP5 had a highly conserved Asn-Pro-Ala (NPA) region and aromatic/arginine (ar/R) motif and it belongs to Nodulin 26-like intrinsic protein (NIP) group II. Analysis of gene expression indicated that CiNIP5 expressed mainly in the roots of citrus seedlings, and it increased obviously under B-deficiency but was suppressed under B toxicity. Additionally, the responses of CiNIP5 expression to B deficiency were compared between the roots of Carrizo citrange and Fragrant citrus, in which the former showed more tolerance than the latter to low B condition in field practice. CiNIP5 transcript level in the root of Carrizo citrange was induced and increased continuously to 7.7 times at 48 h after B deficient treatment (HABdT) as compared with that at 0 HABdT while in the root of Fragrant citrus it was induced to 4.4 times at 24 HABdT, and then decreased to half at 48 HABdT. The possible role of CiNIP5 was discussed in the mechanism of B-uptake efficiency of different rootstocks under B-deficiency.
机译：据报道，在缺乏B的条件下，AtNIP5; 1导致硼（B）吸收到根中。在这项研究中，我们从三叶草橙中克隆了一个类似NIP5的基因（名为CiNIP5）。 CiNIP5的全长为1470 bp，其中903 bp构成了开放阅读框，编码300个氨基酸的蛋白质。序列分析表明，CiNIP5具有高度保守的Asn-Pro-Ala（NPA）区和芳族/精氨酸（ar / R）模体，属于Nodulin 26样内在蛋白（NIP）组II。基因表达分析表明，CiNIP5主要在柑橘幼苗的根部表达，在缺硼条件下CiNIP5明显增加，但在B毒性下却受到抑制。此外，比较了Carrizo柑橘和香柑橘的根中CiNIP5表达对B缺乏的响应，在野外实践中，前者对低B条件表现出更大的耐受性。 B缺陷处理（HABdT）后48 h诱导Carrizo橙子根中的CiNIP5转录水平，与0 HABdT相比持续增加至7.7倍，而香柑橘根中24 HABdT时诱导4.4倍。 ，然后降至48 HABdT的一半。讨论了CiNIP5在缺硼条件下不同砧木的B吸收效率机制中的可能作用。

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