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首页> 外文期刊>Scandinavian journal of clinical and laboratory investigation. >Strong negative interference of ethamsylate (Dicynone?) in serum creatinine quantification via enzymatic assay using Trinder reaction
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Strong negative interference of ethamsylate (Dicynone?) in serum creatinine quantification via enzymatic assay using Trinder reaction

机译：使用Trinder反应的酶法测定法中乙胺磺酸（Dicynone？）对血清肌酐定量的强烈负面影响

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Background. With discrepancies encountered as early as the verification of enzymatic method for quantification of serum creatinine, our research pointed to a later confirmed interference caused by a compound called ethamsylate present in the commonly used antihemorrhagic drug Dicynone. Methods. We measured concentrations of creatinine of 10 patients with blood taken before and 15 minutes after the intravenous administration of a 500 mg dose of Dicynone. The creatinine concentration was determined using Jaffe method and enzymatic method that utilize Trinder reaction (Roche) in analyzer Cobas c 501 (Roche AG, Basel, Switzerland). We also monitored concentration of blood creatinine in three patients before and 15 minutes after application of Dicynone (500 mg i.v.) and in the following 6th, 12th, 18th, and 24th hours. Results. We discovered a significant negative bias in creatinine results using enzymatic assay with Trinder reaction in blood taken 15 min after i.v. application of 500 mg Dicynone to patients compared to their pre-application values (average decrease of 47%). Unlike this, the results of compensated Jaffe method yielded steady results in all samples (average deviation 0.6% from original values). However, 12 h after the drug administration comparable results were seen as before the administration. Conclusion. Considering the strong negative interference of ethamsylate in enzymatic assay using Trinder reaction for creatinine quantification, blood from patients with prescribed Dicynone should be taken at least 12 h after the last application of the drug for obtaining the correct creatinine values.
机译：背景。早在酶法定量血清肌酐的验证中就遇到了差异，我们的研究指出，后来证实的干扰是由常用抗出血药地西酮中存在的名为乙胺酯的化合物引起的。方法。我们测量了静脉注射500 mg地西酮之前和之后15分钟采血的10名患者的肌酐浓度。使用Jaffe法和酶法，在分析仪Cobas c 501（Roche AG，巴塞尔，瑞士）中利用Trinder反应（Roche）确定肌酸酐浓度。我们还监测了在使用Dicynone（500 mg i.v.）之前和之后15分钟以及随后的第6、12、18和24小时中三名患者的血肌酐浓度。结果。我们在静脉注射15分钟后的血液中使用Trinder反应进行酶促测定，发现肌酐结果存在显着的负偏差。与患者使用前的值相比，使用500 mg地西酮的患者（平均减少47％）。与此不同的是，补偿Jaffe方法的结果在所有样本中均得到稳定的结果（与原始值的平均偏差为0.6％）。然而，在给药后12小时，可观察到与给药前相当的结果。结论。考虑到乙苯甲酸酯在使用Trinder反应进行肌酐定量的酶促测定中具有强烈的负干扰作用，应在最后一次应用该药物后至少12小时抽取处方药Dicynone患者的血液，以获取正确的肌酐值。

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