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首页> 外文期刊>Seed Science and Technology >A less lethal sodium hydroxide test for determining seed coat colour in wheat
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A less lethal sodium hydroxide test for determining seed coat colour in wheat

机译：致命性较低的氢氧化钠试验，用于测定小麦的种皮颜色

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摘要

Seed coat colour is an important trait in wheat and helps define market class, grade and value of a crop. Current low-technology seed certification tests to check seed coat colour rely on a sodium hydroxide (NaOH) soak which magnifies visual differences between red and white seeds. Unfortunately, NaOH is a strong base and when imbibed by the seed, germination is reduced, limiting further genetic or phenotypic inspection of individual seeds. Here, a modified, less lethal NaOH test is reported. Suggested modifications to the standardised test are a reduced NaOH solution temperature of 21 degrees C and a seed soak time of two minutes, followed by immediate rinsing of seeds under running water. For validation, known red and white wheat kernels were examined visually, by percentage germination and by principle component analysis (PCA) of red, green and blue (RGB) colour values for both standard and modified NaOH soak procedures. The modified test procedure still allows accurate visual, colour-based identification of 'off-type' seeds, and allows germination and DNA extraction so seed certification decisions may be validated by further investigation
机译：种皮颜色是小麦的重要特征，有助于确定农作物的市场等级，等级和价值。当前用于检查种皮颜色的低技术种子认证测试依赖于氢氧化钠（NaOH）浸泡，这会放大红色和白色种子之间的视觉差异。不幸的是，NaOH是强碱，当种子吸收时，发芽减少，从而限制了单个种子的进一步遗传或表型检查。此处报道了改良的，致死性较低的NaOH测试。建议对标准测试进行的修改是将NaOH溶液温度降低至21摄氏度，将种子浸泡时间缩短为两分钟，然后在流水下立即冲洗种子。为了验证，通过标准的和改良的NaOH浸泡程序，通过发芽百分比和红色，绿色和蓝色（RGB）颜色值的主成分分析（PCA），目测检查已知的红色和白色小麦粒。修改后的测试程序仍然可以基于视觉，基于颜色的方式准确识别“异型”种子，并且可以进行发芽和DNA提取，因此可以通过进一步调查来验证种子认证决定

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