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首页> 外文期刊>Biomaterials >Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering
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Injectable biodegradable hydrogel composites for rabbit marrow mesenchymal stem cell and growth factor delivery for cartilage tissue engineering

机译：用于兔骨髓间充质干细胞和生长因子的软骨组织工程注射用可生物降解的水凝胶复合材料

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We investigated the development of an injectable, biodegradable hydrogel composite of oligo(poly(ethylene glycol) fumarate) (OPF) with encapsulated rabbit marrow mesenchymal stem cells (MSCs) and gelatin microparticles (MPs) loaded with transforming growth factor-β1 (TGF-β1) for cartilage tissue engineering applications. Rabbit MSCs and TGF-β1-loaded MPs were mixed with OPF, a poly(ethylene glycol)-diacrylate crosslinker and the radical initiators ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine, and then crosslinked at 37 ℃ for 8 min to form hydrogel composites. Three studies were conducted over 14 days in order to examine the effects of: (1) the composite formulation, (2) the MSC seeding density, and (3) the TGF-β1 concentration on the chondrogenic differentiation of encapsulated rabbit MSCs. Bioassay results showed no significant difference in DNA amount between groups, however, groups with MPs had a significant increase in glycosaminoglycan content per DNA starting at day 7 as compared to controls at day 0. Chondrocyte-specific gene expression of type II collagen and aggrecan were only evident in groups containing TGF-β1-loaded MPs and varied with TGF-β1 concentration in a dose-dependent manner. Specifically, type II collagen gene expression exhibited a 161±49-fold increase and aggrecan gene expression a 221±151-fold increase after 14 days with the highest dose of TGF-β1 (16 ng/ml). These results indicate that encapsulated rabbit MSCs remained viable over the culture period and differentiated into chondrocyte-like cells, thus suggesting the potential of OPF composite hydrogels as part of a novel strategy for localized delivery of stem cells and bioactive molecules.
机译：我们研究了可注射，可生物降解的寡聚（聚（乙二醇）富马酸酯）（OPF）与封装的兔骨髓间充质干细胞（MSC）和明胶微粒（MPs）负载转化生长因子-β1（TGF- β1）用于软骨组织工程应用。将兔MSC和TGF-β1负载的MP与OPF，聚乙二醇-二丙烯酸酯交联剂和自由基引发剂过硫酸铵和N，N，N'，N'-四甲基乙二胺混合，然后在37℃下交联8分钟形成水凝胶复合材料。为了研究以下因素，在14天内进行了三项研究：（1）复合制剂，（2）MSC接种密度，以及（3）TGF-β1浓度对包囊兔MSC软骨分化的影响。生物测定结果表明，各组之间的DNA量没有显着差异，但是，与MPs组相比，第0天从第7天开始，每个DNA的糖胺聚糖含量显着增加。II型胶原和聚集蛋白聚糖的软骨细胞特异性基因表达为仅在含有TGF-β1的MPs组中明显，并且随TGF-β1浓度的变化呈剂量依赖性。具体而言，以最高剂量的TGF-β1（16 ng / ml）在14天后，II型胶原基因表达增加了161±49倍，聚集蛋白聚糖基因表达增加了221±151倍。这些结果表明，包封的兔间充质干细胞在培养期间仍保持活力，并分化为软骨样细胞，因此表明OPF复合水凝胶的潜力作为局部递送干细胞和生物活性分子的新策略的一部分。

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