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Can chlorophyll fluorescence be used to determine the optimal time to harvest rice seeds for long-term genebank storage?

机译:是否可以使用叶绿素荧光来确定收获水稻种子以长期保存种质库的最佳时间?

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Chlorophyll fluorescence (CF) analysis was explored as a potential tool to identify the optimal time to harvest rice seed germplasm for maximum storage longevity. Seeds of 20 diverse genebank accessions were harvested at 24, 31, 38 and 45d after peak flowering (DAF) and half of each seed lot was sorted by hand, following normal practice at the T.T. Chang Genetic Resources Center. CF analysis was carried out on both non-sorted and sorted seeds, while storage experiments were carried out on sorted seeds. Seed longevity (the time for viability to fall to 50%, p(50)) was significantly correlated with the skewness, kurtosis, mode and mean of the CF histograms when the data for every accession at all the harvest times were included in the correlation analysis. However, these correlation coefficients were 0.481. The correlation coefficient between p(50) and DAF was similarly low (0.461). For individual accessions, there was wide variation in the correlation coefficients. While for some accessions, there appeared to be a strong relationship between p(50) and mean CF that could be used to guide when to harvest seeds in the field, for other accessions, a unique mean CF to inform when to harvest seeds or for use in seed sorting could not be identified; this was also true for DAF over the harvesting schedule used in this study. Given the number and diversity of accessions managed by a genebank, it seems unlikely that CF analysis would be an appropriate tool to help manage the regeneration or processing of seeds intended for storage in the genebank.
机译:叶绿素荧光(CF)分析被认为是一种潜在的工具,可用来确定最佳收割水稻种子种质的时间,以最大程度地延长存储寿命。按照T.T. Chang遗传资源中心的常规做法,在高峰开花(DAF)后第24、31、38和45天收获20种不同种质库种质的种子,并手工分选每个种子批次的一半。对未分选和分选的种子均进行了CF分析,而对分选的种子进行了储藏实验。当将所有收获时间的每个种质的数据都包括在相关性中时,种子寿命(存活率下降到50%的时间,p(50))与CF直方图的偏度,峰度,众数和均值显着相关。分析。但是,这些相关系数为0.481。 p(50)与DAF之间的相关系数同样很低(0.461)。对于单个登录,相关系数差异很大。对于某些种质,p(50)与平均CF之间似乎存在很强的关系,可用于指导何时在田间收获种子,对于其他种质,唯一的平均CF可以告知何时收获种子或用于无法确定用于种子分选的用途;对于本研究中使用的收获时间表中的DAF而言也是如此。考虑到种质库管理的种质的数量和多样性,CF分析似乎不太可能成为一种合适的工具,以帮助管理旨在储存在种质库中的种子的再生或加工。

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