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首页> 外文期刊>Central European Journal of Chemistry >Determination of active components in Chinese medicinal preparations by capillary electrophoresis
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Determination of active components in Chinese medicinal preparations by capillary electrophoresis

机译：毛细管电泳法测定中药制剂中的有效成分

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摘要

The determination of paeoniforin,paeonol,and censenoside Rg1 in traditional Chinese medicinal preparations,Tze Po San Pien pills and Liuwen Dihuang pills has been investigated by micellar electrokinetic capillary electrophoresis with borate buffer(20 mM),sodium dodecylsulfate(30 mM)and acetonitrile(20%)as background electrolytes(pH 9.30),20 kV applied voltage and 203 nm UV detection. The effects of SDS concentration,borate,buffer pH,and organic modifier on electrophoretic behavior and separation are discussed.Regression equations revealed linear relationships between the peak-area of each component and the content with the correlation coeffcients from 0.9982 to 0.9999.In addition, the levels of the active compounds in two kinds of traditional Chinese medicinal preparations were easily determined with the recoveries from 93.1%to 108.2%.
机译：胶束电动毛细管电泳-硼酸缓冲液（20 mM），十二烷基硫酸钠（30 mM）和乙腈（乙醛）的研究探讨了中药制剂，慈波三片丸和六文地黄丸中of药中的for药苷，eon药酚和皂苷Rg1的含量20％）作为背景电解质（pH 9.30），20 kV施加电压和203 nm紫外检测。讨论了SDS浓度，硼酸根，缓冲液pH值和有机改性剂对电泳行为和分离的影响。回归方程揭示了各组分的峰面积与含量之间的线性关系，相关系数在0.9982至0.9999之间。两种中药制剂中活性成分的含量很容易测定，回收率在93.1％至108.2％之间。

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