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首页> 外文期刊>Separation and Purification Technology >Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A
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Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A

机译：通过用Cibacron Blue F3G-A修饰的Span 85反向胶束亲和萃取进行蛋白质分离

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摘要

The reversed micellar solution formed by Sorbitan trioleate (Span 85) coupled with Cibacron Blue F3G-A (CB) as an affinity ligand show good potential for use in reversed micellar protein extraction operations.In this work,the extraction behavior of lysozyme and ovalbumin with the reversed micellar phase has been investigated at extensive pH values and CB concentrations.The partitioning isotherms of lysozyme and ovalbumin were expressed by the Langmuir equation.By addition of 3 vol.% hexanol to the reversed micellar phase,the extraction capacity of lysozyme reached 6.38 mg/mL,over seven times higher than that of ovalbumin (0.83 mg/mL).Thus,lysozyme was separated from crude chicken ovalbumin solutions with a purification factor over 20.Moreover,the reversed micellar solution was recycled three times for the separation,and the purification factor was kept nearly 20 in the recycling.Incomplete back extraction of lysozyme to the stripping solution compromised the total recovery yields in the following recycles,so more effective back extraction method needs to be developed to make the extraction system more attractive.In general,the present results have shown the potential of the affinity-based reversed micellar system for application in protein separations.
机译：三油酸山梨糖醇酯（Span 85）与Cibacron Blue F3G-A（CB）作为亲和配体形成的反向胶束溶液具有良好的潜力，可用于反向胶束蛋白质的提取操作。在这项工作中，溶菌酶和卵清蛋白的提取行为在Langmuir方程中表示了溶菌酶和卵清蛋白的分配等温线。通过在胶束相中加入3％（体积）的己醇，溶菌酶的提取能力达到了6.38。 mg / mL，是卵清蛋白（0.83 mg / mL）的7倍以上。因此，从鸡卵清蛋白粗品溶液中分离了溶菌酶，纯化系数超过20。此外，反向胶束溶液循环了3次用于分离，回收过程中纯化因子保持在20左右。在回收之后，需要开发更有效的反萃取方法以使萃取系统更具吸引力。总的来说，目前的结果表明基于亲和力的逆胶束系统在蛋白质分离中的潜力。

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