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Supercritical technology as an alternative to fractionate prebiotic galactooligosaccharides

机译:超临界技术可替代分离益生元低聚半乳糖

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摘要

In the present work, supercritical technology based on carbon dioxide has been used as an alternative to fractionate galactooligosaccharides (GOS) in a dairy-based prebiotic ingredient. The main challenge of the fractionation of the commercial mixture is the combination of GOS with monosaccharides and disaccha-rides. Considering that the beneficial health effects of the commercial mixture, such as the improvement of the immune system, have been associated to its GOS content, therefore, a purification and/or removal of other sugars can be of great importance.The goal of this study was focused in the fractionation of the complex carbohydrate mixture according to the degree of polymerization of the different carbohydrates. Supercritical carbon dioxide (SC-CO2) with different ethanol/water mixtures as co-solvents has been tested for the selective recovery of the prebiotic carbohydrates. The appropriate selection of the co-solvent employed, together with the most suitable extraction conditions (including temperature, pressure and co-solvent flow rate) allowed the almost complete removal of monosaccharides and disaccharides from the mixture and lead to a residue in the extraction cell where mainly tri- and tetra-saccharides were present with ca. 75 wt% of purity and 94% recovery.
机译:在当前的工作中,基于二氧化碳的超临界技术已被用作分馏基于乳制品的益生元成分中的低聚半乳糖(GOS)的替代方法。商业混合物的分离的主要挑战是GOS与单糖和二糖的结合。考虑到市售混合物的有益健康影响(例如免疫系统的改善)与其GOS含量有关,因此,其他糖类的纯化和/或去除可能非常重要。根据不同碳水化合物的聚合程度,重点研究了复杂碳水化合物混合物的分馏。已对具有不同乙醇/水混合物作为助溶剂的超临界二氧化碳(SC-CO2)进行了选择性回收益生元碳水化合物的测试。对所用助溶剂的适当选择以及最合适的萃取条件(包括温度,压力和助溶剂流速)使混合物中的单糖和二糖几乎完全去除,并在萃取池中产生残留物其中主要存在三糖和四糖纯度为75 wt%,回收率为94%。

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