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Polypropylene silanized membranes for immunoisolation

机译：聚丙烯硅烷化膜用于免疫隔离

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摘要

Transplantation of cells secreting biologically active substances like cytokines,hormones or growth factors,may be a way of patient treatment in deficiency of these factors.Application of the permiselective barrier is the method of separation of the transplanted cells from the host cells to prevent the immune rejection.A review of polypropylene silanized hollow fiber membranes applications for immunoisolation of cells is presented.We have elaborated modified polypropylene membranes and applied them as the systems for isolation of cells.Modification of membranes by silanization allowed us to avoid tissue overgrowth during the implantation into mice.The applied membranes exhibited a stable cut-off value during about 3 months implantation into animal.The spectroscopic evaluation of absorption for red irradiation (FTIR) of the membrane material,revealed no differences in chemical structure before and after the implantation.The membranes were applied for isolation of hybridoma OKT3 cells secreting monoclonal antibody IgG2a reactive to human T lymphocytes,human parathyroid cells producing parathormon,WEHI-3B mouse cell line producing IL-3 (involved in the hematopoietic stem cells differentiation).Our separating systems were constructed with idea for immunoprotection of graft,for delivering of parathormon in hypoparathyroidism or for supporting of haematopoiesis.Summarizing,the elaborated membranes evaluated in the in vitro experiments and in the in vivo short animal experiments have turned out promising as systems for the local production of biologically active substances.
机译：在缺乏这些因子的情况下，移植分泌诸如细胞因子，激素或生长因子等生物活性物质的细胞可能是患者治疗的一种方法。渗透选择性屏障的应用是将移植细胞与宿主细胞分离以防止免疫的方法。聚丙烯硅烷化中空纤维膜在细胞免疫分离中的应用综述，我们已经详细阐述了改性聚丙烯膜并将其作为细胞分离系统的应用。通过硅烷化对膜进行修饰可以避免植入过程中组织过度生长。所植入的膜在植入动物体内约3个月后表现出稳定的截止值。对膜材料的红光吸收（FTIR）进行光谱学评估，发现植入前后的化学结构无差异。被用于分离杂交瘤OKT3细胞creting单克隆抗体IgG2a与人T淋巴细胞反应，产生人甲状旁腺的副甲状腺细胞，产生IL-3的WEHI-3B小鼠细胞系（参与造血干细胞的分化）。总之，在体外实验和体内短动物实验中评价的精心制作的膜已被证明可作为本地生产生物活性物质的系统。

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