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首页> 外文期刊>Separation and Purification Technology >Separation and purification of DL-tetrahydropalmatine from Corydalis yanhusuo by high-speed counter-current chromatography
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Separation and purification of DL-tetrahydropalmatine from Corydalis yanhusuo by high-speed counter-current chromatography

机译：高速逆流色谱法分离延胡索中DL-四氢巴马汀

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摘要

High-speed counter-current chromatography(HSCCC)was applied to preparative separation and purification of DL-tetrahydropalmatine from Corydalis yanhusuo by a one-step separation.The two-phase system consisted of petroleum ether-ethyl acetate-methanol-water(15:30:21:20,v/v)was employed.An orthogonal design was used for optimizing the revolution speed of the separation column,flow rate of the mobile phase and separation temperature,which was 850 rpm,1.2ml/min and 20 °C,respectively.HPLC analysis of the fractions collected by preparative HSCCC of 200 mg of crude extracts showed that the purity of DL-tetrahydropalmatine(8.6 mg)was 96.4%.The chemical identity of the component was confirmed by ~1H NMR and EI-MS.
机译：高速逆流色谱（HSCCC）用于一步法从延胡索延胡索中制备DL-四氢巴马汀的制备分离和分离。两相系统由石油醚-乙酸乙酯-甲醇-水（15： 30：21：20，v / v）。采用正交设计优化分离柱的转速，流动相流速和分离温度，分别为850 rpm，1.2ml / min和20°制备型HSCCC收集的200 mg粗提物的馏分的HPLC分析表明，DL-四氢巴马汀（8.6 mg）的纯度为96.4％。〜1H NMR和EI-证实了该组分的化学性质多发性硬化症。

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