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首页> 外文期刊>Separation and Purification Technology >Development and optimization of a novel simultaneous microwave-assisted extraction and adsorbent treatment process for separation and recovery of paclitaxel from plant cell cultures
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Development and optimization of a novel simultaneous microwave-assisted extraction and adsorbent treatment process for separation and recovery of paclitaxel from plant cell cultures

机译：开发和优化新型微波辅助萃取和吸附剂处理工艺从植物细胞培养物中分离和回收紫杉醇

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摘要

A novel simultaneous microwave-assisted extraction (MAE) and adsorbent treatment process was developed for recovering the anticancer agent paclitaxel from plant cell cultures and removing plant-derived tar and waxy compounds. The efficiency of the process was maximized by optimizing several major process variables. The optimal extraction time, ratio of adsorbent to liquid-liquid extract, ratio of methanol to biomass and extraction temperature were found to be 6 min, 4.5:1 (w/w), 1:1 (v/w) and 40 °C, respectively. By performing the simultaneous process under these conditions, we were able to recover most of the paclitaxel (>99%) with a purity of ～22% after hexane precipitation. The efficiency of this process is similar to that of the conventional process (purity: ～20%, yield: ～95%) in which paclitaxel recovery is separate from adsorbent treatment. Therefore, it is expected that the conventional process can be simplified by simultaneously performing paclitaxel recovery and removal of impurities, thus dramatically reducing the cost of separation/purification.
机译：为了从植物细胞培养物中回收抗癌药紫杉醇并去除植物来源的焦油和蜡状化合物，开发了一种新颖的同时微波辅助萃取（MAE）和吸附剂处理工艺。通过优化几个主要的过程变量，使过程效率最大化。最佳萃取时间，吸附剂与液-液萃取物的比例，甲醇与生物质的比例以及萃取温度分别为6分钟，4.5：1（w / w），1：1（v / w）和40°C ， 分别。通过在这些条件下同时进行处理，我们能够在己烷沉淀后以〜22％的纯度回收大部分紫杉醇（> 99％）。该方法的效率与常规方法（纯度：〜20％，产率：〜95％）相似，紫杉醇的回收与吸附剂的处理是分开的。因此，期望可以通过同时进行紫杉醇的回收和杂质的去除来简化常规方法，从而显着降低分离/纯化的成本。

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