Purification of lumbrokinase from Eisenia fetida using aqueous two-phase systems and anion-exchange chromatography

N.B. Iannucci; S.A. Camperi; O. Cascone

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Purification of lumbrokinase from Eisenia fetida using aqueous two-phase systems and anion-exchange chromatography

机译：使用水相两相系统和阴离子交换色谱法纯化费氏艾氏菌中的激酶

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An 8.1% polyethyleneglycol (PEG) 300/22.2% phosphate aqueous two-phase system was used to obtain a clear extract of the earthworm Eisenia fetida. Lumbrokinase was then purified from the extract by anion-exchange chromatography at pH 4.6.80% enzyme activity was recovered with a purification factor of 8.0 and 97% purity. No further purification was achieved by submitting the final product to chromatography on benzamidine-agarose.

机译：使用8.1％的聚乙二醇（PEG）300 / 22.2％的磷酸盐水溶液两相系统获得aEisenia fetida的澄清提取物。然后通过阴离子交换色谱法在pH 4.6.80％的条件下从提取物中纯化出Lumbrokinase。回收的酶活度为8.0，纯度为97％。通过将最终产物在苯甲idine-琼脂糖上进行色谱，无法进一步纯化。

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《Separation and Purification Technology》 |2008年第1期|共4页
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N.B. Iannucci; S.A. Camperi; O. Cascone;
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正文语种 eng
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Aqueous two-phase systems; Earthworm; Eisenia fetida; Fibrinolytic; Lumbrokinase; Thrombolytic;

机译：水两相系统;th;赤藓;纤维蛋白溶解;肺激酶;血栓溶解;

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机译：使用水相两相系统和阴离子交换色谱法纯化费氏艾氏菌中的激酶
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Purification of lumbrokinase from Eisenia fetida using aqueous two-phase systems and anion-exchange chromatography

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