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首页> 外文期刊>Separation and Purification Technology >The isolation and characterization of polysaccharides from longan pulp
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The isolation and characterization of polysaccharides from longan pulp

机译：龙眼肉中多糖的分离与表征

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摘要

Crude water-soluble longan polysaccharides (LPSs) were extracted from longan pulp using hot water assisted by microwave pretreatment and ethanol precipitation. Neutral and acidic polysaccharides were separated by DEAE-cellulose anion-exchange chromatography. High performance gel permeation chromatography (HPGPC) analysis showed that the average molecular weight (M_w) of neutral polysaccharide (LPS-N) was approximately 13.8 kDa, and those of two acidic polysaccharides (LPS-A1 and LPS-A2) were approximately 1382 and 571 kDa, respectively. Structural properties and compositions of these three LPS were examined by FTIR and HPLC. It was hypothesized that LPS belong to P-type acidic heteropolysac-charides with pyran group, among which LPS-N was composed of xylose and glucose at the molar ratio of 1:1.9; LPS-A1 consisted of rhamnose, xylose, arabinose and galactose at the molar ratio of 1:1.64:4.33:2.28; and LPS-A2 comprised only rhamnose. The total uronic acid content of LPS-A1 and LPS-A2 were 6% and 19%, respectively.
机译：在微波预处理和乙醇沉淀辅助下，用热水从龙眼肉中提取粗制水溶性龙眼多糖（LPS）。通过DEAE-纤维素阴离子交换色谱分离中性和酸性多糖。高效凝胶渗透色谱（HPGPC）分析表明，中性多糖（LPS-N）的平均分子量（M_w）约为13.8 kDa，两种酸性多糖（LPS-A1和LPS-A2）的平均分子量（M_w）约为1382和分别为571 kDa。通过FTIR和HPLC检查这三种LPS的结构性质和组成。假设LPS属于具有吡喃基的P型酸性杂多糖，其中LPS-N由木糖和葡萄糖组成，摩尔比为1：1.9。 LPS-A1由鼠李糖，木糖，阿拉伯糖和半乳糖组成，摩尔比为1：1.64：4.33：2.28; LPS-A2仅包含鼠李糖。 LPS-A1和LPS-A2的总糖醛酸含量分别为6％和19％。

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