• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Separation and Purification Technology >Optimization of high-speed counter-current chromatography for separation of polyphenols from the extract of hawthorn (Crataegus laevigata) with response surface methodology
【24h】

Optimization of high-speed counter-current chromatography for separation of polyphenols from the extract of hawthorn (Crataegus laevigata) with response surface methodology

机译：响应面分析法优化山楂提取物中多酚的高速逆流色谱法分离

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Separation and purification of polyphenols from the extract of hawthorn (Crataegus laevigata) was investigated with high-speed counter-current chromatography (HSCCC). Solvent systems were examined according to partition coefficients of target components and n-butanol-ethyl acetate-water (1:1:2, v/v/v) system was chosen. The critical experimental parameters of HSCCC were optimized and determined with response surface methodology (RSM) as following: Revolution speed was 850 rpm, flow-rate was 1.5 mL/min and temperature was 25 °C. Under these conditions, 500 mg of crude extract was introduced into the HSCCC system and a product of 9.7 mg with purity over 94.8% was obtained in 260 min, which was identified as chlorogenic acid with UV, LC-MS and ~1H NMR The result proved that RSM is an efficient method to optimize HSCCC conditions, and HSCCC could be a very effective approach to separate and purify chlorogenic acid from natural source.
机译：用高速逆流色谱法（HSCCC）研究了山楂（Crataegus laevigata）提取物中多酚的分离和纯化。根据目标组分的分配系数检查溶剂系统，并选择正丁醇-乙酸乙酯-水（1：1：2，v / v / v）系统。对HSCCC的关键实验参数进行了优化，并通过响应面方法（RSM）确定如下：转速为850 rpm，流速为1.5 mL / min，温度为25°C。在这些条件下，将500 mg粗提取物引入HSCCC系统，在260 min内获得纯度为94.8％的9.7 mg产品，经UV，LC-MS和〜1H NMR鉴定为绿原酸。证明RSM是优化HSCCC条件的有效方法，而HSCCC可能是从天然来源分离和纯化绿原酸的非常有效的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号