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首页> 外文期刊>Separation and Purification Technology >Green extraction by aqueous two-phase systems of porcine pancreatic and snake venom phospholipase A(2)
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Green extraction by aqueous two-phase systems of porcine pancreatic and snake venom phospholipase A(2)

机译：猪胰腺和蛇毒磷脂酶A（2）的水两相系统绿色萃取

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摘要

Phospholipases comprise a group of enzymes with applications in the thermal stability and emulsifying capacity of food formulations. In this work aqueous two-phase systems (ATPS) were used to extract phospholipase A(2) (PLA(2)) from raw porcine pancreas and snake venom at 25 degrees C. PLA(2) was partitioned into ATPS composed of inorganic salts, water and polyethylene glycol. We determined the partition coefficient, recovery factor, purification factor, enzyme activity and emulsifying activity for the enzyme extracted with the different ATPS tested. The highest partition coefficient (K-p = 81.7) and the highest theoretical recovery percentage of PLA(2) in the top and bottom phases of the systems were obtained from the pancreas extract. The highest recovery percentage in the top phase was obtained in a system with PEG1500/Na2SO4/H2O. Pearson correlation analysis between the ATPS descriptor parameters and the recovery and partitioning parameters of PLA(2) showed significant, albeit small, correlation between molar mass and PEG phase volume. Furthermore, Principal Component Analysis showed that the best system for PLA(2) purification was with ATPS PEG1500/Na2SO4/H2O. In addition to preserving enzyme activity, the ATPS provided a high partition coefficient of PLA(2) (81.67%) and can be considered a viable technique for pre-purification of PLA(2). (C) 2014 Elsevier B.V. All rights reserved.
机译：磷脂酶包含一组酶，这些酶可用于食品配方的热稳定性和乳化能力。在这项工作中，使用水性两相系统（ATPS）在25摄氏度下从生猪胰腺和蛇毒中提取磷脂酶A（2）（PLA（2））。PLA（2）分成由无机盐组成的ATPS ，水和聚乙二醇。我们确定了用不同测试ATPS提取的酶的分配系数，回收率，纯化率，酶活性和乳化活性。从胰腺提取物中获得了系统最高相和最低相中最高的分配系数（K-p = 81.7）和最高的理论回收率（2）。在具有PEG1500 / Na2SO4 / H2O的系统中，顶层得到了最高的回收率。在ATPS描述符参数与PLA（2）的回收和分配参数之间的Pearson相关分析显示，摩尔质量与PEG相体积之间具有显着的相关性，尽管很小。此外，主成分分析显示PLA（2）纯化的最佳系统是使用ATPS PEG1500 / Na2SO4 / H2O。除了保留酶活性外，ATPS还提供了PLA（2）的高分配系数（81.67％），可以认为是预纯化PLA（2）的可行技术。 （C）2014 Elsevier B.V.保留所有权利。

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