Stem cell enrichment approaches.

Park PC; Selvarajah S; Bayani J; Zielenska M; Squire JA

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Stem cell enrichment approaches.

机译：干细胞富集方法。

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Adult somatic tissue, and the tumours that arise therein, are maintained by a small population of stem cells. In addition to the self-renewal potential and pluripotency, these stem cells express several phenotypic traits that can be used in isolation and enrichment strategies. Since most of the traits are not exclusive to the stem cells however, the resultant populations are typically heterogeneous and variable from one isolation to another. In this article, we review the strategies for isolation of stem cells, and the limitations thereof, with emphasis on mesenchymal tissue and bone tumours. The emerging evidence suggests that stem cell is not a distinct entity, but rather an indefinite state along a spectrum, characterized by phenotypic traits, epigenetic factors and the microenvironment.

机译：成年的体细胞组织以及其中产生的肿瘤由少量的干细胞维持。除了自我更新的潜力和多能性外，这些干细胞还表达了几种表型性状，可用于分离和富集策略。然而，由于大多数特征并非干细胞所独有，因此所得群体通常是异质的，并且从一个分离株到另一个分离株是可变的。在本文中，我们回顾了分离干细胞的策略及其局限性，重点是间充质组织和骨肿瘤。新兴证据表明，干细胞不是一个独特的实体，而是沿谱线的不确定状态，其特征在于表型性状，表观遗传因素和微环境。

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《Seminars in cancer biology》 |2007年第3期|共8页
作者
Park PC; Selvarajah S; Bayani J; Zielenska M; Squire JA;
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正文语种 eng
中图分类肿瘤学;
关键词
Stem Cells; trait; isolation aspects; Tissues; 干细胞; 组织;

机译：Stem Cells;trait;isolation aspects;Tissues;干细胞;组织;

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专利

1. Stem cell enrichment approaches. [J] . Park PC, Selvarajah S, Bayani J, Seminars in cancer biology . 2007,第3期

机译：干细胞富集方法。
2. Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system. [J] . Dong R, Liu X, Liu Y, Cell biology international. . 2007,第7期

机译：通过快速粘附富集表皮干细胞，并使用有机型系统分析表皮干细胞与相邻细胞的相互相互作用。
3. Pitfalls of Cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX): Existing Dead Cells During In Vitro Selection Anticipate the Enrichment of Specific Aptamers [J] . Avci-Adali Meltem, Metzger Markus, Perle Nadja, Oligonucleotides . 2010,第6期

机译：配体通过指数富集（SELEX）进行细胞系统进化的陷阱：体外选择过程中现有的死细胞预期特定适体的富集。
4. Extracellular Matrix Enriched Spheroids for Enhanced Survival and Differentiation of Mesenchymal Stem Cells [C] . Tomas Gonzalez-Fernandez, Andrew T. Keown, Alejandro J. Tenorio, Society for Biomaterials annual meeting and exposition . 2019

机译：细胞外基质富集的球体增强间充质干细胞的存活和分化。
5. Enrichment of therapeutic hematopoietic stem cell populations from embryonic stem cells. [D] . Gilner, Jennifer Bushman. 2007

机译：从胚胎干细胞中富集治疗性造血干细胞群体。
6. Bone Mesenchymal Stem Cell-Enriched β-Tricalcium Phosphate Scaffold Processed by the Screen-Enrich-Combine Circulating System Promotes Regeneration of Diaphyseal Bone Non-Union [O] . Xin Wang, WenXiang Chu, YiFu Zhuang, 2019

机译：筛选富集联合循环系统处理的骨髓间充质干细胞富集的β-磷酸三钙支架促进骨干骨不愈合的再生
7. Analysis and prediction of pathways in HeLa cells by integrating biological levels of organization with systems-biology approaches. [O] . Juan Carlos Higareda-Almaraz, Ilse A Valtierra-Gutiérrez, Magdalena Hernandez-Ortiz, 2013

机译：通过将生物组织水平与系统生物学方法相结合，分析和预测HeLa细胞中的通路。

Stem cell enrichment approaches.

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