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首页> 外文期刊>Biomaterials >In vitro reaction to orthopaedic biomaterials by macrophages and lymphocytes isolated from patients undergoing revision surgery.
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In vitro reaction to orthopaedic biomaterials by macrophages and lymphocytes isolated from patients undergoing revision surgery.

机译：从接受翻修手术的患者中分离出的巨噬细胞和淋巴细胞对骨科生物材料的体外反应。

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Periprosthetic tissues observed at sites of loose total joint implants exhibit abundant macrophages, lymphocytes, fibroblasts and particulate debris. Macrophages phagocytose orthopaedic debris and release proinflammatory cytokines, chemokines, matrix metalloproteinases and other substances. In addition, other cell types present in tissues harvested from the bone-implant interface are thought to influence periprosthetic bone resorption. The present study examined the effects of polymethylmethacrylate (PMMA), cobalt chrome molybdenum alloy (CoCr), and titanium-alloy particle challenge on macrophages co-cultured with lymphocytes in vitro. Potential synergistic effects of lymphocytes on macrophage activation were determined by measuring interleukin-6 and tumor necrosis factor-alpha release following exposure to orthopaedic biomaterial particles. Exposure of macrophages or macrophages co-cultured with lymphocytes to all three types of particles resulted in increased release of interleukin-6 and tumor necrosis factor-alpha at 48 h, when compared to macrophages or macrophages co-cultured with lymphocytes, respectively, cultured in the absence of particles. Lymphocytes isolated from periprosthetic tissues secreted increased basal levels of cytokines relative to peripheral blood lymphocytes. Higher doses of PMMA and titanium-alloy particles stimulated increased levels of cytokine release in the macrophage and macrophage/lymphocyte groups. In contrast, a higher dose of CoCr particles (0.075% v/v) was not as effective as the 0.015% v/v dose, indicating probable CoCr toxicity. The macrophage/lymphocyte co-culture did not show synergism between the two types of cells with respect to cytokine release. T-cells at the bone-implant interface may alter the biological response to particulate debris.
机译：在松散的全关节植入物部位观察到的假肢周围组织表现出丰富的巨噬细胞，淋巴细胞，成纤维细胞和微粒碎片。巨噬细胞吞噬整形外科碎片并释放促炎细胞因子，趋化因子，基质金属蛋白酶和其他物质。另外，认为存在于从骨-植入物界面收获的组织中的其他细胞类型会影响假体周围的骨吸收。本研究研究了聚甲基丙烯酸甲酯（PMMA），钴铬钼合金（CoCr）和钛合金颗粒对巨噬细胞与淋巴细胞共培养的影响。暴露于整形外科生物材料颗粒后，通过测量白介素6和肿瘤坏死因子-α释放来确定淋巴细胞对巨噬细胞激活的潜在协同作用。与分别与淋巴细胞共培养的巨噬细胞或巨噬细胞相比，在48 h暴露于巨噬细胞或与淋巴细胞共培养的巨噬细胞暴露于所有三种类型的颗粒会导致白细胞介素6和肿瘤坏死因子-α的释放增加。没有颗粒。从假体周围组织分离的淋巴细胞分泌的基础细胞因子水平相对于外周血淋巴细胞增加。更高剂量的PMMA和钛合金颗粒刺激了巨噬细胞和巨噬细胞/淋巴细胞组中细胞因子释放水平的提高。相反，较高剂量的CoCr颗粒（0.075％v / v）不如0.015％v / v剂量有效，表明可能存在CoCr毒性。巨噬细胞/淋巴细胞共培养在细胞因子释放方面没有显示出两种类型的细胞之间的协同作用。骨植入物界面处的T细胞可能会改变对颗粒碎片的生物反应。

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