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首页> 外文期刊>ORL: Journal for oto-rhino-laryngology and its borderlands >High prevalence of Streptococcus pneumoniae with mutations in pbp1a, pbp2x, and pbp2b genes of penicillin-binding proteins in the nasopharynx in children in Japan.
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High prevalence of Streptococcus pneumoniae with mutations in pbp1a, pbp2x, and pbp2b genes of penicillin-binding proteins in the nasopharynx in children in Japan.

机译：日本儿童鼻咽青霉素结合蛋白的pbp1a，pbp2x和pbp2b基因突变的肺炎链球菌患病率很高。

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OBJECTIVE: To evaluate the resistances of Streptococcus pneumoniae to beta-lactams developed by stepwise alterations in high-molecular-weight penicillin-binding proteins (PBPs) with a reduced binding affinity of beta-lactams. Among the numerous mutations in pbp genes that alter the affinity for beta-lactams, the decreased affinity of PBP1A, 2X and 2B is especially important in the development of resistances to beta-lactams. STUDY DESIGN: Retrospective review. METHODS: In this study, we investigated the mutations in pbp1a, pbp2x, and pbp2b genes evaluated by polymerase chain reaction (PCR) in 866 pneumococcal isolates collected from the nasopharynx of Japanese children with acute otitis media. RESULTS: 210 strains (24.3%) exhibited no mutations in the three pbp genes. 333 strains (38.5%) had mutations in the three pbp genes, 78 (9.0%) in two pbp genes, whereas 245 (28.3%) displayed mutations in only one pbp gene. Among the 656 strains with mutations in pbp genes, 620 (94.5%) strains had mutations in pbp2x. The annual prevalence of antimicrobial-resistant S. pneumoniae showed a gradual increase in strains with mutations in the three pbp genes and a parallel decrease in strains without mutations. CONCLUSIONS: PCR-based genotyping can characterize the antimicrobial resistances in pneumococci along with minimal inhibitory concentrations (MICs). Physicians should pay attention to the recent increase in antimicrobial-resistant S. pneumoniae when treating pediatric acute otitis media.
机译：目的：评估肺炎链球菌对β-内酰胺结合亲和力降低的高分子量青霉素结合蛋白（PBPs）进行逐步改变而产生的对β-内酰胺的耐药性。在pbp基因的众多突变中，它们改变了与β-内酰胺的亲和力，其中PBP1A，2X和2B的亲和力下降对抵抗β-内酰胺的发展尤为重要。研究设计：回顾性审查。方法：在这项研究中，我们调查了聚合酶链反应（PCR）评估的866株肺炎球菌分离株中pbp1a，pbp2x和pbp2b基因的突变，这些分离株是从日本患有急性中耳炎儿童的鼻咽中收集的。结果：210个菌株（24.3％）在三个pbp基因中均未显示出突变。 333个菌株（38.5％）在三个pbp基因中有突变，两个菌株中有78个（9.0％）在两个pbp基因中有突变，而245个菌株（28.3％）在一个pbp基因中显示出突变。在656个pbp基因突变的菌株中，有620个（94.5％）菌株的pbp2x突变。耐药性肺炎链球菌的年度流行率表明，三个pbp基因突变的菌株逐渐增加，而无突变菌株的平行减少。结论：基于PCR的基因分型可以表征肺炎球菌的抗菌素耐药性以及最小抑菌浓度（MIC）。在治疗小儿急性中耳炎时，医师应注意近期耐药性肺炎链球菌的增加。

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