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首页> 外文期刊>Soil Science and Plant Nutrition >Improved tolerance to boron deficiency by enhanced expression of the boron transporter BOR2
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Improved tolerance to boron deficiency by enhanced expression of the boron transporter BOR2

机译：通过增强硼转运蛋白BOR2的表达提高对硼缺乏的耐受性

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Boron (B) cross-links the pectin polysaccharide rhamnogalacturonan II (RG-II) and thus is important for cell wall structure in plants. B deficiency is an agricultural problem that causes significant losses of crop productivity worldwide. To address this, B deficiency-tolerant plants have been generated using B transporters. With the goal of further improving plant tolerance to low-B conditions, we generated transgenic Arabidopsis thaliana (L.) Heynh. with enhanced expression of BOR2, a B transporter that promotes cross-linking of RG-II and root elongation under low B supply. We generated a DNA construct containing the cauliflower mosaic virus 35S RNA promoter, a native promoter of BOR2, a BOR2 gene and green fluorescent protein (GFP) (Pro35S-BOR2: BOR2-GFP), and obtained three independent transgenic lines with relatively high levels of BOR2-GFP expression. In the transgenic lines, BOR2-GFP was expressed mainly in the lateral root caps in the meristem zone and in the epidermal cells in the elongation zone, similar to its expression when driven only by its native promoter. In the Pro35S-BOR2: BOR2-GFP lines, BOR2-GFP was also expressed in various cells in the maturation zone of roots and epidermal cells of shoots, where the expression was hardly detectable in ProBOR2: BOR2-GFP lines. The transgenic lines were cultured under various B concentrations on solid media and it was found that root growth of three lines and shoot growth of two lines were enhanced compared to wild-type plants under low-B conditions. This finding established that enhanced expression of BOR2 leads to improved root growth under low-B conditions. We also examined growth of the transgenic lines under hydroponic conditions. One of the three lines showed better growth and fertility under a low-B condition, while the wild type did not set seeds under the same condition, suggesting the potential utility of BOR2 expression in agricultural applications.
机译：硼（B）使果胶多糖鼠李糖半乳糖醛酸聚糖II（RG-II）交联，因此对于植物的细胞壁结构很重要。硼缺乏症是一个农业问题，会导致全球范围内作物生产力的重大损失。为了解决这个问题，已经使用B转运蛋白产生了耐B缺乏植物。为了进一步提高植物对低B条件的耐受性，我们产生了转基因拟南芥（L.）Heynh。 B转运蛋白BOR2的表达增强，B转运蛋白在低B供给下促进RG-II的交联和根伸长。我们生成了一个包含花椰菜花叶病毒35S RNA启动子，BOR2天然启动子，BOR2基因和绿色荧光蛋白（GFP）（Pro35S-BOR2：BOR2-GFP）的DNA构建体，并获得了三个相对较高水平的独立转基因品系BOR2-GFP的表达。在转基因品系中，BOR2-GFP主要在分生组织区的侧根盖和延伸区的表皮细胞中表达，类似于仅由其天然启动子驱动时的表达。在Pro35S-BOR2：BOR2-GFP品系中，BOR2-GFP也表达在根的成熟区和芽的表皮细胞中的各种细胞中，在ProBOR2：BOR2-GFP品系中几乎检测不到该表达。在不同的B浓度下在固体培养基上培养转基因品系，发现与低B条件下的野生型植物相比，三个品系的根生长和两个品系的枝条生长得到增强。该发现确定了在低B条件下增强的BOR2表达导致根生长的改善。我们还研究了在水培条件下转基因品系的生长。在低B条件下，三株系之一显示出更好的生长和育性，而野生型在相同条件下未结种，表明BOR2表达在农业应用中具有潜在的效用。

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