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首页> 外文期刊>Soil Research >Development of a phosphatase activity assay using excised plant roots.
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Development of a phosphatase activity assay using excised plant roots.

机译：使用切除的植物根部开发磷酸酶活性测定法。

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Root phosphatase mediated mineralisation of organic phosphorus (P) can affect P availability in agricultural and forest landscapes. Phosphatases hydrolyse organic P into inorganic P that can be taken up by plants. We developed a method to determine mineralisable organic P by phosphatases exuded by excised live roots/microbial systems. We used excised greenhouse- and field-grown roots with para-nitrophenylphosphate, glucose-1-phosphate and phytic acid as sources of organic P. Experimental variables were analysed including linearity of the reaction, presence of inorganic P, organic P exuded from roots, possible abiotic degradation of organic P, and background inorganic/organic P. Organic P mineralisation by root-phosphatase complexes was found to be linear through 6h. Phosphorus contaminants into the system were found to be within 10% of mineralised organic P. We used this technique to answer questions about organic P bioavailability, including effect of organic P sources, plant species, plant variety, plant stress and root conditions. Overall, this method was sensitive to organic P source and plant stress of greenhouse and field-grown roots, plant species and root physiological conditions. Unlike other methods used to determine phosphatase activity, this method is not limited by lengthy preparation to develop model plants, nor is there any restriction on the choice of organic P or plant species. Our results suggest that this is an attractive method for determining organic P mineralisation specificity among and within plant species, and it can be easily integrated into routine laboratory analyses.
机译：根磷酸酶介导的有机磷矿化可以影响农业和森林景观中磷的有效性。磷酸酶将有机磷水解为植物可以吸收的无机磷。我们开发了一种方法，可以通过切除的活根/微生物系统渗出的磷酸酶来确定可矿化的有机磷。我们使用经过割割的温室和田间种植的根系，以及对硝基苯基磷酸酯，葡萄糖-1-磷酸和植酸作为有机磷的来源。分析了实验变量，包括反应的线性，无机磷的存在，从根中渗出的有机磷，可能是有机磷和背景无机/有机磷的非生物降解。根磷酸酶复合物对有机磷的矿化作用一直持续到6h。发现进入系统的磷污染物在矿化有机磷的10％以内。我们使用该技术回答了有关有机磷生物利用度的问题，包括有机磷源，植物种类，植物品种，植物胁迫和根系状况的影响。总体而言，该方法对温室和田间根系的有机磷源和植物胁迫，植物种类和根系生理状况敏感。与用于确定磷酸酶活性的其他方法不同，此方法不受开发模型植物的冗长准备的限制，对有机磷或植物种类的选择也没有任何限制。我们的结果表明，这是一种确定植物种间和植物内有机磷矿化特异性的有吸引力的方法，可以轻松地将其整合到常规实验室分析中。

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