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首页> 外文期刊>Spanish Journal of Agricultural Research >Role of Bradyrhizobium japonicum induced by genistein on soybean stressed by water deficit
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Role of Bradyrhizobium japonicum induced by genistein on soybean stressed by water deficit

机译：金雀异黄素诱导的日本根瘤菌对缺水胁迫的大豆的作用

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摘要

The soybean (Glycine max (L.) Merr.) is a crop mainly grown under rain fed conditions although irrigation is increasingly being used. Water deficiency is the main factor limiting seed production. The symbiosis process is also negatively affected by water stress. The isoflavone genistein have been recognized as a powerful inducer of Nod factors production by Bradyrhizobium and its addition to inocula has been shown to increase nodule number and promote soybean nitrogen (N) fixation at low temperatures. This study looks for answers about the possible role of genistein in countering the stress on nodulation produced by water deficit in soybeans. Bradyrhizobium japonicum SEMIA 5079 was grown in culture media induced or not induced with genistein. Inocula were applied to plants growing at different moisture levels. The effect of the treatments on nodulation and N content was evaluated. An improved response to drought stress was observed when the bacteria were grown in presence of genistein as a Nod factors inducer. Nodulation values under moisture stress differed from 8.9 nodules plant(-1) with genistein at 10 mu M to 1.8 nodules plant(-1) when no inducer was used. Genistein reduced the negative effect on nodulation caused by water deficiency.
机译：大豆（Glycine max（L.）Merr。）是一种主要在雨养条件下生长的作物，尽管越来越多地使用灌溉。缺水是限制种子产量的主要因素。水分胁迫也对共生过程产生负面影响。异黄酮金雀异黄素已被认为是由缓生根瘤菌产生Nod因子的强大诱导剂，其在接种物中的添加已显示出可增加根瘤数并在低温下促进大豆固氮。这项研究寻找有关染料木黄酮可能在抵抗大豆水分亏缺所致结瘤压力方面可能发挥作用的答案。延缓根瘤菌SEMIA 5079在染料木黄酮诱导或未诱导的培养基中生长。将接种物应用于以不同水分含量生长的植物。评估了处理对结瘤和氮含量的影响。当细菌在染料木黄酮作为Nod因子诱导剂的情况下生长时，观察到对干旱胁迫的响应有所改善。在不使用诱导剂的情况下，水分胁迫下的结瘤值与含染料木黄酮的8.9个结节植物（-1）和10μM的1.8个结节植物（-1）不同。金雀异黄素减少了缺水对结瘤的负面影响。

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