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首页> 外文期刊>Spine >Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc.
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Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc.

机译：转导的牛关节软骨细胞在体外影响共培养的髓核细胞的代谢：对软骨细胞移植到椎间盘的影响。

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STUDY DESIGN: Biologic study on the effects of coculture of bovine articular chondrocytes transduced ex vivo with genes expressing bone morphogenetic proteins (BMPs) on nucleus pulposus (NP) cells. OBJECTIVE: To evaluate the effects of bovine articular chondrocytes transduced with adenoviruses expressing various BMPs on proteoglycan and collagen production, and cellular proliferation of NP cells in vitro. SUMMARY OF BACKGROUND DATA: Matrix synthesis by intervertebral disc cells is promoted by exposing the cells to growth factors or delivering genes that permit sustained expression of growth factors. We propose a novel therapeutic approach involving delivery of autologous chondrocytes, transduced ex vivo with bioactive proteins, to provide both the cells and proteins required to stimulate disc healing. METHODS: Adult bovine articular chondrocytes were transduced with adenoviruses (Ads) expressing either BMP-2, 4, 5, 7, 10, or 13 and plated as monolayers. Bovine NP cells encapsulated in alginate beads were cocultured, floating in the medium. Proteoglycan and collagen accumulation, and NP cell proliferation were measured after 6 days of coculture. As a positive control, beads were cocultured with articular chondrocytes in the presence of rhBMP-7. RESULTS: NP cells cocultered with articular chondrocytes transduced with BMPs-2, 4, 7, and 10 accumulated significantly (P < 0.05) more proteoglycan than when cocultured with chondrocytes transduced with AdGFP (control) [AdBMP-2: 23.6%; AdBMP-4: 27.0%; AdBMP-7: 129.1%; AdBMP-10: 102.1% increases respectively]. Collagen accumulation was significantly (P < 0.05) increased by NP cells cocultured with articular chondrocytes transduced with BMPs-2, 4, 5, and 7. [AdBMP-2: 104.6%; AdBMP-4: 40.6%; AdBMP-5: 58.6%; AdBMP-7: 55.5% increases respectively]. NP cells proliferated when cocultured with articular chondrocytes transduced with AdBMP-2 and -7. CONCLUSIONS: Bovine NP cells are stimulated to produce proteoglycans and collagen when exposed to chondrocytes transduced with genes for various BMPs. If applied to the treatment of disc degeneration, this strategy could provide the disc with not only metabolically active chondrocytes but also promote matrix replenishment by stimulating native NP cells.
机译：研究设计：生物学研究牛离体软骨细胞与表达骨形态发生蛋白（BMPs）的基因共转染对髓核（NP）细胞的影响。目的：研究表达多种BMP的腺病毒转导的牛关节软骨细胞对蛋白聚糖和胶原蛋白的产生以及NP细胞体外增殖的影响。背景数据概述：通过使椎间盘细胞暴露于生长因子或递送允许生长因子持续表达的基因来促进椎间盘细胞的基质合成。我们提出了一种新颖的治疗方法，涉及通过生物活性蛋白离体转导的自体软骨细胞的递送，以提供刺激椎间盘愈合所需的细胞和蛋白。方法：用表达BMP-2、4、5、7、10或13的腺病毒（Ads）转导成年牛关节软骨细胞，并单层铺板。将封装在藻酸盐珠中的牛NP细胞共培养，使其漂浮在培养基中。共培养6天后，测量蛋白聚糖和胶原蛋白的积累，以及NP细胞的增殖。作为阳性对照，在rhBMP-7存在下，将小球与关节软骨细胞共培养。结果：与由BMPs-2、4、7和10转导的关节软骨细胞共培养的NP细胞比与由AdGFP转导的软骨细胞共培养时（对照）的蛋白聚糖积聚（P <0.05）[AdBMP-2：23.6％; AdBMP-4：27.0％; AdBMP-7：129.1％; AdBMP-10：分别增加102.1％]。 NP细胞与BMPs-2、4、5和7转导的关节软骨细胞共培养后，胶原蛋白的积聚显着增加（P <0.05）。[AdBMP-2：104.6％; p> 0.05。 AdBMP-4：40.6％; AdBMP-5：58.6％; AdBMP-7：分别增加55.5％]。与AdBMP-2和-7转导的关节软骨细胞共培养时，NP细胞增殖。结论：当暴露于各种BMPs基因转导的软骨细胞中时，牛NP细胞被刺激产生蛋白聚糖和胶原蛋白。如果应用于椎间盘退变的治疗，该策略不仅可以为椎间盘提供具有代谢活性的软骨细胞，而且还可以通过刺激天然NP细胞促进基质的补充。

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