...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Spine >The adeno associated viral vector as a strategy for intradiscal gene transfer in immune competent and pre-exposed rabbits.
【24h】

The adeno associated viral vector as a strategy for intradiscal gene transfer in immune competent and pre-exposed rabbits.

机译：腺相关病毒载体作为免疫能力强和暴露前兔子的椎间盘内基因转移的策略。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

STUDY DESIGN: Experimental animal study. OBJECTIVES: This study evaluates the in vitro and in vivo transduction efficacy and transgene expression in immune competent and pre-exposed rabbits. SUMMARY OF BACKGROUND DATA: Degenerative disc disease (DDD) continues to pose a substantial clinical problem. Therapeutic options such as an interbody fusion are highly invasive and result in the loss of the intervertebral disc. In addition, interbody fusion puts the adjacent discs at an even higher risk for disc degeneration. A novel approach to slow DDD is to introduce high levels of growth factors into the degenerating disc by delivering the gene coding for the appropriate growth factor. The most efficient technique to do so to date uses viral vectors. However, viral vectors may be problematic because of their immunogenicity. The adeno-associated virus (AAV) viral vector is known to be less immunogenic than commonly used adenoviral vectors. METHODS: Human nucleus pulposus cells were transduced in vitro. Twenty-four Rabbits were injected with AAV viral vectors carrying different marker genes. Transgene expression and the humoral/cellular immune response to the vector was evaluated. RESULTS: We could show that the AAV viral vector transduces human as well as rabbit nucleus pulposus cells in vitro and in vivo. There is a significant humoral immune response against the AAV vector that decreases transgene expression over 10-fold in preimmunized animals. CONCLUSIONS: AAV is a valuable new vector to achieve transgene expression in the intervertebral disc. In preimmunized animals, its use needs to be further evaluated because of the significant reduction in transgene expression.
机译：研究设计：实验动物研究。目的：本研究评估了具有免疫能力的和预先暴露的兔子的体外和体内转导功效以及转基因表达。背景数据概述：退行性椎间盘疾病（DDD）继续构成重大的临床问题。诸如椎间融合的治疗选择具有很高的侵入性，并导致椎间盘丢失。另外，椎间融合使相邻椎间盘处于更高的椎间盘退变风险。减慢DDD的新方法是通过传递编码适当生长因子的基因，将高水平的生长因子引入退化的椎间盘。迄今为止，最有效的方法是使用病毒载体。然而，由于其免疫原性，病毒载体可能是有问题的。已知腺相关病毒（AAV）病毒载体的免疫原性低于常用的腺病毒载体。方法：体外转导人髓核细胞。向24只兔注射携带不同标记基因的AAV病毒载体。评价了转基因表达和对该载体的体液/细胞免疫应答。结果：我们可以证明AAV病毒载体在体外和体内都能转导人以及兔髓核细胞。针对AAV载体存在显着的体液免疫反应，可使预免疫动物中的转基因表达降低10倍以上。结论：AAV是实现转基因在椎间盘中表达的有价值的新载体。在预免疫动物中，由于转基因表达显着降低，因此需要进一步评估其用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号