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首页> 外文期刊>Spine >Comparative effects of bone morphogenetic proteins and Sox9 overexpression on matrix accumulation by bovine anulus fibrosus cells: implications for anular repair.
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Comparative effects of bone morphogenetic proteins and Sox9 overexpression on matrix accumulation by bovine anulus fibrosus cells: implications for anular repair.

机译：骨形态发生蛋白和Sox9过表达对牛肛门纤维细胞基质积累的比较作用：对肛门修复的影响。

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STUDY DESIGN: An in vitro biologic study comparing the effects of a series of bone morphogenetic proteins (BMPs) and Sox9 on the extracellular matrix accumulation by bovine anulus fibrosus (AF) cells. OBJECTIVE: To compare the effects of adenoviral-mediated overexpression of various BMPs and Sox9 on extracellular matrix accumulation by AF cells in vitro. SUMMARY OF BACKGROUND DATA: Repair of the disrupted AF, which is perceived to be a potential therapy to diminish nucleus pulposus (NP) herniation, may also offer a treatment strategy for severe symptomatic degenerative disc disease. To date, no systematic comparison of a large group of growth factors in the AF has been published. In this study, we compared the effects of the adenoviral-mediated overexpression of 12 BMPs and Sox9 on extracellular matrix production by AF cells. METHODS: Adult monolayer-cultured bovine AF cells were transduced with adenoviral vectors containing human BMP and green fluorescence protein (GFP) genes (AdBMPs), or Sox9 and GFPgenes (AdSox9), or GFP gene alone (AdGFP, as negative control). Proteoglycan and collagen accumulation, and cell proliferation were measured for each of the treatment groups 6 days after viral transduction. RESULTS: AF cells transduced with BMP-2, -3, -5, -7, -8, -12, -13, -14, and -15, and Sox9 accumulated significantly more collagen than AF cells transduced with AdGFP (control). AF cells transduced with AdBMP-2, -4, -7, -10, -12, and -13, and AdSox9 accumulated significantly more proteoglycans than AF cells transduced with AdGFP. CONCLUSION: We have demonstrated the relative effectiveness of 12 different BMPs and Sox9 on the stimulation of proteoglycan and/or collagen accumulation by AF cells. This study is the first to compare the relative effectiveness of various BMPs and Sox9 on extracellular matrix accumulation by AF. This information should prove useful to those seeking to develop a strategy for repair of the AF in humans.
机译：研究设计：一项体外生物学研究，比较了一系列骨形态发生蛋白（BMP）和Sox9对牛肛门纤维（AF）细胞胞外基质积累的影响。目的：比较腺病毒介导的多种BMP和Sox9的过量表达对AF细胞体外细胞外基质积累的影响。背景数据概述：破坏性房颤的修复，被认为是减少髓核（NP）疝的潜在疗法，也可以为严重的症状性退行性椎间盘疾病提供治疗策略。迄今为止，尚未发表房颤中大量生长因子的系统比较。在这项研究中，我们比较了腺病毒介导的12 BMP和Sox9对AF细胞产生的细胞外基质的影响。方法：用含有人BMP和绿色荧光蛋白（GFP）基因（AdBMPs）或Sox9和GFP基因（AdSox9）或单独GFP基因（AdGFP作为阴性对照）的腺病毒载体转导成人单层培养的牛AF细胞。病毒转导后6天，对每个治疗组测量蛋白聚糖和胶原蛋白的积累以及细胞增殖。结果：BMP-2，-3，-5，-7，-8，-12，-13，-14和-15转导的AF细胞和Sox9积累的胶原蛋白明显多于AdGFP转导的AF细胞（对照） 。用AdBMP-2，-4，-7，-10，-12和-13以及AdSox9转导的AF细胞比用AdGFP转导的AF细胞积聚更多的蛋白聚糖。结论：我们已经证明了12种不同的BMP和Sox9对AF细胞刺激蛋白聚糖和/或胶原积累的相对有效性。这项研究是首次比较各种BMP和Sox9对AF引起的细胞外基质累积的相对有效性。该信息对于那些寻求制定人类房颤修复策略的人应该是有用的。

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