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首页> 外文期刊>Cephalalgia >Platelet-activating factor (PAF) in internal jugular venous blood of migraine without aura patients assessed during migraine attacks.
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Platelet-activating factor (PAF) in internal jugular venous blood of migraine without aura patients assessed during migraine attacks.

机译：在偏头痛发作期间评估没有先兆患者的偏头痛颈内静脉血中的血小板活化因子（PAF）。

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Sarchielli P, Alberti A, Coppola F, Baldi A, Gallai B, Floridi Ar, Floridi Al, Capocchi G & Gallai V. Platelet-activating factor (PAF) in internal jugular venous blood of migraine without aura patients assessed during migraine attacks. Cephalalgia 2004; 24:623-630. London. ISSN 0333-1024The aim of the study was to verify the production of PAF and the activity of PAF acetyl-hydrolase (PAF-AH), the enzyme involved in the catabolism of this phospholipid mediator, in migraine attacks. Their levels were determined during migraine crises in serial samples of internal jugular venous blood taken from five migraine patients without aura, who were admitted to the hospital during the crises. Internal jugular venous blood samples were taken immediately after catheter insertion at 1, 2, and 4 h after attack onset, and within 2 h from its cessation. PAF was purified by high-performance liquid chromatography (HPLC) and determined by radioimmunoassay method. The enzymatic activity of PAF-AH was measured by reverse-phase HPLC, based on the derivatization with 7-diethylaminocoumarin-3-carbonylazide. In the internal jugular venous blood of migraine patients without aura (MO), an increase was observed in PAF levels, which was already evident at the time of catheter insertion (885.6 +/- 82.8) and at the first hour (868.4 +/- 65.24) (Anova: P < 0.0001). PAF levels remained elevated through the second (746.8 +/- 82.95), fourth (700.6 +/- 34.93) and sixth hours (644.4 +/- 42.85), and then decreased at the end of the attack, reaching levels significantly lower than those measured at the time of catheter insertion (565.5 +/- 38.34). The activity of PAF-AH showed an opposite trend with higher values at the first hour and significantly lower values at the second and fourth hours from the beginning of the migraine attack (Anova: P < 0.02). The increased production of PAF may account for persistent platelet activation during migraine crises, even in the presence of an increased production of nitric oxide (NO) end-products which, on the other hand, should instead intervene in counteracting and limiting platelet activation. Potential sources of PAF production are the endothelial cells from cerebral vessels, stimulated by trigeminal neuropeptides, platelets themselves, and mast cells, as suggested by the neurogenic inflammation model.
机译：Sarchielli P，Alberti A，Coppola F，Baldi A，Gallai B，Floridi Ar，Floridi Al，Capocchi G和Gallai V.偏头痛发作期间评估无先兆患者偏头痛内部颈静脉血中的血小板活化因子（PAF）。头肌痛2004; 24：623-630。伦敦。 ISSN 0333-1024该研究的目的是验证偏头痛发作中PAF的产生以及PAF乙酰水解酶（PAF-AH）的活性，PAF乙酰水解酶与该磷脂介质的分解代谢有关。在偏头痛危机期间，从五名无先兆偏头痛患者的一系列颈内静脉血样本中确定了它们的水平，这些患者在危机期间入院。在发作开始后1、2和4小时，以及在停止后2小时内，立即在插入导管后采集颈内静脉血样。 PAF通过高效液相色谱法（HPLC）纯化，并通过放射免疫分析法测定。通过用7-二乙基氨基香豆素-3-羰基叠氮化物衍生化，通过反相HPLC测量PAF-AH的酶活性。在没有先兆（MO）的偏头痛患者的颈内静脉血中，PAF水平升高，这在导管插入时（885.6 +/- 82.8）和第一小时（868.4 +/-）已经很明显。 65.24）（方差分析：P <0.0001）。 PAF水平在第二（746.8 +/- 82.95），第四（700.6 +/- 34.93）和第六小时（644.4 +/- 42.85）内一直保持升高，然后在攻击结束时降低，达到的水平明显低于那些在插入导管时测量（565.5 +/- 38.34）。 PAF-AH的活性呈相反的趋势，从偏头痛发作开始的第一个小时开始，其值较高，而第二和第四个小时则明显较低（Anova：P <0.02）。 PAF产生的增加可能解释了偏头痛危机期间持续的血小板活化，即使存在一氧化氮（NO）终产物产生增加的情况，另一方面，NO最终产物应干预和限制血小板的活化。 PAF产生的潜在来源是三叉神经肽，血小板自身和肥大细胞刺激的脑血管内皮细胞，如神经性炎症模型所暗示的那样。

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