...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >The effect of the degree of chitosan deacetylation on the efficiency of gene transfection.
【24h】

The effect of the degree of chitosan deacetylation on the efficiency of gene transfection.

机译：壳聚糖脱乙酰度对基因转染效率的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chitosans with various degrees of deacetylation were synthesized by acetylation with acetic anhydride. These chitosans were evaluated for efficacy of nanoparticle formation, DNA binding efficiency, morphology, and in vitro and in vivo gene transfection efficiency. DNA binding efficacy was reduced as degree of deacetylation was decreased, therefore requiring an increased +/-ratio to effect complete DNA complexation. For chitosan with a molecular weight of 390kDa, the +/-ratio to achieve complete DNA complexation for degrees of deacetylation of 90%, 70% and 62% was 3.3:1, 5.0:1, and 9.0:1, respectively. The size and morphology of these nanoparticle formulations were not significantly different. The decreased degree of deacetylation results in a decrease in overall luciferase expression levels in HEK293, HeLa, and SW756 cells due to particle destabilization in the presence of serum proteins. However, intramuscular luciferase expression levels increased with decreasing deacetylation over the time points tested. Degree of chitosan deacetylation is an important factor in chitosan-DNA nanoparticle formulation as it affects DNA binding, release and gene transfection efficiency in vitro and in vivo.
机译：通过用乙酸酐乙酰化来合成具有不同脱乙酰度的壳聚糖。评估了这些壳聚糖的纳米颗粒形成功效，DNA结合效率，形态以及体外和体内基因转染效率。 DNA结合功效随脱乙酰基度的降低而降低，因此需要增加+/-比率才能实现完全的DNA络合。对于分子量为390kDa的壳聚糖，对于90％，70％和62％的脱乙酰度，实现完全DNA络合的+/-比分别为3.3：1、5.0：1和9.0：1。这些纳米颗粒制剂的大小和形态没有显着差异。脱乙酰化程度的降低导致HEK293，HeLa和SW756细胞中总体荧光素酶表达水平的降低，这是由于存在血清蛋白的情况下颗粒不稳定造成的。但是，在测试的时间点内，肌内萤光素酶表达水平随着脱乙酰作用的降低而增加。壳聚糖脱乙酰化程度是壳聚糖-DNA纳米颗粒制剂中的重要因素，因为它会影响体内外的DNA结合，释放和基因转染效率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号