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首页> 外文期刊>Stem Cells >Toward engineering a human neoendothelium with circulating progenitor cells.
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Toward engineering a human neoendothelium with circulating progenitor cells.

机译：致力于利用循环祖细胞改造人类新内皮。

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Tissue-engineered vascular grafts may one day provide a solution to many of the limitations associated with using synthetic vascular grafts. However, identifying a suitable cell source and polymer scaffold to recreate the properties of a native blood vessel remains a challenge. In this work, we assess the feasibility of using endothelial progenitor cells (EPCs) found in circulating blood to generate a functional endothelium on poly(1,8-octanediol-co-citrate) (POC), a biodegradable elastomeric polyester. EPCs were isolated from human blood and biochemically differentiated into endothelial-like cells (HE-like) in vitro. The differentiated cell phenotype and function was confirmed by the appearance of the characteristic endothelial cell (EC) cobblestone morphology and positive staining for EC markers, von Willebrand factor, vascular endothelial cadherin, flk-1, and CD31. In addition, HE-like cells cultured on POC express endothelial nitric oxide synthase at levels comparable to aortic ECs. Furthermore, as with mature endothelial cells, HE-like cell populations show negligible expression of tissue factor. Similarly, HE-like cells produce and secrete prostacyclin and tissue plasminogen activator at levels comparable to venous and aortic ECs. When compared to fibroblast cells, HE-like cells cultured on POC show a decrease in the rate of plasma and whole-blood clot formation as well as a decrease in platelet adhesion. Finally, the data show that HE-like cells can withstand physiological shear stress of 10 dynes/cm(2) when cultured on POC-modified expanded poly(tetrafluoroethylene) vascular grafts. Collectively, these data are the foundation for future clinical studies in the creation of an autologous endothelial cell-seeded vascular graft.
机译：组织工程化的血管移植物可能有一天为解决与使用合成血管移植物相关的许多局限性提供解决方案。然而，鉴定合适的细胞来源和聚合物支架以重建天然血管的特性仍然是一个挑战。在这项工作中，我们评估了使用循环血液中发现的内皮祖细胞（EPC）在聚（1,8-辛二醇-柠檬酸酯）（POC）（一种可生物降解的弹性体聚酯）上生成功能性内皮的可行性。 EPCs是从人血中分离出来的，并在体外通过生物化学方法分化为内皮样细胞（HE-like）。通过特征性内皮细胞（EC）鹅卵石形态的出现以及EC标记，von Willebrand因子，血管内皮钙黏着蛋白，flk-1和CD31的阳性染色，证实了分化的细胞表型和功能。此外，在POC上培养的类HE细胞表达的内皮一氧化氮合酶水平与主动脉EC相当。此外，与成熟的内皮细胞一样，HE样细胞群体的组织因子表达可忽略不计。同样，HE样细胞产生和分泌前列环素和组织纤溶酶原激活剂的水平与静脉和主动脉EC相当。与成纤维细胞相比，在POC上培养的类HE细胞的血浆和全血凝块形成速率降低，血小板粘附降低。最后，数据显示当在POC修饰的扩展聚四氟乙烯血管移植物中培养时，HE样细胞可以承受10达因/厘米（2）的生理切应力。总的来说，这些数据是未来自体内皮细胞种血管移植物的临床研究的基础。

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