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首页> 外文期刊>Stem cell reviews and Reports >Enhanced functions of human embryonic stem cell-derived hepatocyte-like cells on three-dimensional nanofibrillar surfaces.
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Enhanced functions of human embryonic stem cell-derived hepatocyte-like cells on three-dimensional nanofibrillar surfaces.

机译：人类胚胎干细胞衍生的肝细胞样细胞在三维纳米原纤维表面的功能增强。

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Human embryonic stem cell (hESC)-derived hepatocytes provide a promising unlimited resource for the treatment of liver disease. However, current protocols for the generation of mature and functional hepatocytes are inefficient. Therefore, in order to better differentiate and maintain the function of differentiating hESCs, we have hypothesized that hESCs undergo better differentiation into hepatocyte-like cells (HLCs) when induced on three-dimensional nanofibrillar surfaces. We have demonstrated that, during stepwise differentiation of induction, the markers of hepatic lineage expressed and finally lead to the generation of functional mature cells. In the presence of an ultraweb nanofiber, HLCs produced lower AFP, greater urea, glycogen storage, metabolic PROD activity, uptake of LDL and organic anion ICG, all of which are indicative of the differentiation of HLCs. These results show that topographically treated hESCs at the nano level have a distinct hepatic functionality profile which has implications for cell therapies.
机译：人胚胎干细胞（hESC）衍生的肝细胞为肝病的治疗提供了有希望的无限资源。然而，目前用于产生成熟和功能性肝细胞的方案是无效的。因此，为了更好地区分和维持分化的hESC的功能，我们假设当在三维纳米原纤维表面上诱导时，hESC可以更好地分化为类肝细胞（HLC）。我们已经证明，在诱导的逐步分化过程中，肝谱系的标志物表达并最终导致功能成熟细胞的产生。在超网状纳米纤维的存在下，HLC产生的AFP降低，尿素，糖原存储，代谢PROD活性，LDL的吸收和有机阴离子ICG的吸收降低，所有这些都表明了HLC的分化。这些结果表明，在纳米水平上经地形处理的hESC具有明显的肝功能特征，这对细胞疗法具有重要意义。

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