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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Structural studies of E-coli ribosomes by spectroscopic techniques: A specialized review
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Structural studies of E-coli ribosomes by spectroscopic techniques: A specialized review

机译：光谱技术对大肠杆菌核糖体的结构研究：专门综述

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摘要

We present a review on our interdisciplinary line of research based on strategies of molecular biology and biophysics. These have been applied to the study of the prokaryotic ribosome of the bacterium Escherichia coli. Our investigations on this organelle have continued for more than a decade and we have adopted different spectroscopic biophysical techniques such as: dielectric and fluorescence spectroscopy as well as light scattering (photon correlation spectroscopy). Here we report studies on the whole 70S ribosomes and on the separated subunits 30S and 50S. Our results evidence intrinsic structural features of the subunits: the small shows a more "floppy" structure, while the large one appears to be more rigid. Also, an inner "kernel" formed by the RNA/protein association is found within the ribosome. This kernel is surrounded by a ribonucleoprotein complex more exposed to the solvent. Initial analyses were done on the so called Kaldtschmit-Wittmann ribosome: more recently we have extended the studies to the "tight couple" ribosome known for its better functional performance in vitro. Data evidence a phenomenological correlation between the differential biological activity and the intrinsic structural properties of the two-ribosome species. Finally, investigations were also conducted on particles treated at sub-denaturing temperatures and on ribosomes partially deproteinized by salt treatment (ribosomal cores). Results suggest that the thermal treatment and the selective removal of proteins cause analogous structural alterations. (c) 2005 Elsevier B.V. All rights reserved.
机译：我们提出了基于分子生物学和生物物理学策略的跨学科研究综述。这些已被用于研究大肠杆菌的原核生物核糖体。我们对该细胞器的研究已经持续了十多年，我们采用了不同的光谱生物物理技术，例如：介电和荧光光谱以及光散射（光子相关光谱）。在这里，我们报告了对整个70S核糖体以及分离的30S和50S亚基的研究。我们的结果证明了亚基的内在结构特征：较小的亚基显示出更“松散”的结构，而较大的亚基显示出更刚性。同样，在核糖体内发现了由RNA /蛋白质结合形成的内部“核”。该核被更多暴露于溶剂的核糖核蛋白复合物包围。最初的分析是对所谓的Kaldtschmit-Wittmann核糖体进行的：最近，我们将研究扩展到了以其更好的体外功能表现而闻名的“紧对”核糖体。数据证明了两个核糖体物种的差异生物学活性和内在结构特性之间存在一种现象学相关性。最后，还对在亚变性温度下处理的颗粒以及通过盐处理部分脱蛋白的核糖体（核糖体核心）进行了研究。结果表明热处理和蛋白质的选择性去除引起类似的结构改变。 （c）2005 Elsevier B.V.保留所有权利。

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