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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide
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Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide

机译:十六烷基三甲基溴化铵存在下核酸对钇(III)-芦丁的荧光增强作用

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It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y3+)-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. in hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 x 10(-7) to 1.0 X 10(-5) g/ml for fish sperm DNA (fsDNA), 1.0 x 10(-7) to 4.6 x 10(-6) g/ml for yeast RNA (yRNA), their detection limits (S/N = 3) are 7.5 x 10(-8), 8.0 x 10(-8) g/ml, respectively. The interaction mechanism is also studied. (c) 2005 Elsevier B.V. All rights reserved.
机译:发现在十六烷基三甲基溴化铵(CTMAB)系统存在下,核酸可以增强钇(III)(Y3 +)-芦丁的荧光强度。在六亚甲基四胺(HMTA)-HCl缓冲液中,产生的荧光最大,激发和发射波长分别为452和520 nm。基于此,提出了一种新的荧光测定核酸的方法。在最佳条件下,对于鱼类的精子DNA(fsDNA),增强的荧光强度与核酸浓度在1.0 x 10(-7)至1.0 X 10(-5)g / ml范围内成比例,在1.0 x 10(酵母RNA(yRNA)为-7)至4.6 x 10(-6)g / ml,其检出限(S / N = 3)为7.5 x 10(-8),8.0 x 10(-8)g / ml , 分别。还研究了相互作用机理。 (c)2005 Elsevier B.V.保留所有权利。

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